Immunocytochemistry/ Immunofluorescence abreview for Anti-PIP2 antibody [2C11]

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (24 hr EGF-treated LNCaP Androgen Dependent Prostat)
Specification
24 hr EGF-treated LNCaP Androgen Dependent Prostat
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Dilution
1/50
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS/1% BSA

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor 594 goat anti mouse
Host species: Mouse
Clonality: Monoclonal
Conjugation: Alexa Fluor® 594
Dilution
1/250

Additional data

Additional Notes
Corresponding Hoescht stain for nucleus (blue) is shown.

Skyla Carney

Verified customer

Submitted Oct 19 2011

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