Western blot abreview for Anti-PIP2 antibody [2C11]

Inconclusive
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Loading amount
20 µg
Specification
HEK293
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TTBS

Secondary antibody

Name
Non-Abcam antibody was used: Dako Rabbit Anti Mouse
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000

Detection

Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Positive control
No observed bands detected but after reading your answer to a previous western blot review I see that the antibody is not meant for western blotting as the MW is too small.

Additional data

Abcam response
Thank you for your valuable feedback. We are sorry to hear that this antibody has not given you good results in western blot. This antibody has not been tested in this application before, and it is possible that it may not be suitable for use.

Abcam user community

Verified customer

Submitted Feb 01 2013

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