Overview

  • Product name
  • Description
    Mouse monoclonal to PIP2
  • Host species
    Mouse
  • Specificity
    This antibody cross reacts with Phosphatidic acid (<5.0%).
  • Tested applications
    Suitable for: ICC/IFmore details
  • Immunogen

    Phosphatidylinositol-4,5-biphosphate (PIP2) from bovine spinal cord

Properties

Applications

Our Abpromise guarantee covers the use of ab2335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.

TBS buffer is ideal.

Target

  • Relevance
    Phosphatidylinositol 4,5-biphosphate (PIP2) is a membrane phospholipid that has been implicated in a variety of cellular processes, including synaptic vesicle recycling and signal transduction pathways. PLCD4 hydrolyzes PIP2 to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3).

Images

  • ab2335, staining PIP2 in non-polarized (left) and polarized (right) AX3 cells by Immunocytochemisry/ Immunofluorescence.

    Cells were fixed with formaldehyde, glutaraldehyde, and 0.01% Triton X-100 in PBS for 10 min. Samples were incubated with primary antibody at 1/100 dilution.

References

This product has been referenced in:
  • Wahl S  et al. The Disease Protein Tulp1 Is Essential for Periactive Zone Endocytosis in Photoreceptor Ribbon Synapses. J Neurosci 36:2473-93 (2016). Read more (PubMed: 26911694) »
  • Li J  et al. Actin dynamics is rapidly regulated by the PTEN and PIP2 signaling pathways leading to myocyte hypertrophy. Am J Physiol Heart Circ Physiol 307:H1618-25 (2014). Read more (PubMed: 25260617) »
See all 5 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

Thank you for contacting Abcam

I have talked to the supplier of this antibody and I am sorry but they do not have any detailed clone information that I can share and cannot confirm that this antibody is the same as the one published in the paper indicated.
Sorry that I cannot be of more help in this matter, but if there is anything else I can help you with, please elt me know.

Free RabMab with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

Thank you very much for your call last week, and I'm very sorry for the delay getting back to you.

I've just heard from the lab, and unfortunately ab2335 has not been tested for cross-reactivity with PIP3. The original clone was produced and tested by an outside lab, and we don't have the full ELISA or ICC protocols at this time.

I apologize that we don't have more information about this antibody, but if you have further questions or comments, or if there is anything else that we can do for you, please let me know and I'll be happy to help.

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Question
Answer

Thank you for your enquiry. The cross reactivity of this antibody with other PtdIns is stated below. PIP2 100%; Phosphatidic acid <5.0%; PIP 0.1%; Phosphatidyl inositol <0.2%; Phosphatidyl choline <0.2%; Phosphatidyl serine <0.2%; Phosphatidyl ethanolamine <0.2%; Phosphatidyl glycerol <0.2%; Cardiolipin <0.2%; Cholesterol <0.2%; Diacylglycerol <0.2%

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Question
Answer

Thank you for your enquiry. No ab2335 is not clone 2C11. Please do not hesitate to contact me should you require further assistance.

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Question
Answer

To our knowledge, this antibody has yet to be tested in ICC. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. If you have any additional questions, please contact us again.

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Answer

Thank you for your enquiry, Here are two publications citing the use of this antibody: RIA: Fukami, K., Takenawa, T. (1989) Quantitative Changes in Polysphophoinositides 1,2-diacyglycerol and Inositol 1,4,5-Trisphosphate by Platelet-Derived Growth Factor and Prostaglandin F2a. The Journal of Biological Chemistry 264(25):1485-1489. ELISA: Imai, K., et al. (1999) The Pleckstrin Homology Domain of the Wiskott-Aldrich Syndrome Protein Is Involved in the Organization of Actin Cytoskeleton. Clinical Immunology 92(2):128-137. We do not have any information regarding the cross reactivity to other phosphatidylinositols, my apologies for the inconvenience,

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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