Key features and details
- Rabbit polyclonal to PIP4K2 alpha
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PIP4K2 alpha antibody
DescriptionRabbit polyclonal to PIP4K2 alpha
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- A431, H1299, HeLa and HepG2 whole cell lysate (ab7900). Human thyroid cancer tissue. HeLa cells.
General notesKeep as concentrated solution.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154585 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 46 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
FunctionCatalyzes the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol-4,5-biphosphate.
Tissue specificityExpressed ubiquitously, with high levels in the brain. Present in most tissues, except notably skeletal muscle and small intestine.
Sequence similaritiesContains 1 PIPK domain.
- Information by UniProt
- 1 phosphatidylinositol 4 phosphate 5 kinase 2 alpha antibody
- 1 phosphatidylinositol 4 phosphate 5 kinase antibody
- 1 phosphatidylinositol 4 phosphate kinase antibody
All lanes : Anti-PIP4K2 alpha antibody (ab154585) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 46 kDa
Immunofluorescent analysis of methanol-fixed HeLa cells labeling PIP4K2 alpha with ab154585 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling PIP4K2 alpha with ab154585 at 1/100 dilution.
ab154585 has not yet been referenced specifically in any publications.