Anti-PIP5K1 alpha/PIP5K1A antibody (ab122049)
Key features and details
- Rabbit polyclonal to PIP5K1 alpha/PIP5K1A
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PIP5K1 alpha/PIP5K1A antibody
See all PIP5K1 alpha/PIP5K1A primary antibodies -
Description
Rabbit polyclonal to PIP5K1 alpha/PIP5K1A -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human PIP5K1 alpha/PIP5K1A aa 266-389.
Database link: Q99755 -
Positive control
- Human tonsil tissue; RT-4 and U-251 MG cells lysate; Human cell line A-431
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. Mediates RAC1-dependent reorganization of actin filaments. Acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs. Contributes to the activation of PLD2. -
Tissue specificity
Highly expressed in heart, placenta, skeletal muscle, kidney and pancreas. Detected at lower levels in brain, lung and liver. -
Sequence similarities
Contains 1 PIPK domain. -
Cellular localization
Cell membrane. Endomembrane system. Golgi apparatus > Golgi stack. Nucleus speckle. Associated with the plasma membrane and with internal membranes. Associated with Golgi stacks (By similarity). Detected on RAC1-induced plasma membrane ruffles, and on membrane ruffles induced by platelet-derived growth factor. Localizes to nuclear speckles and associates with TUT1 to regulate polyadenylation of selected mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 8394 Human
- Omim: 603275 Human
- SwissProt: Q99755 Human
- Unigene: 655131 Human
- Unigene: 707569 Human
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Alternative names
- 68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha antibody
- Phosphatidylinositol 4 phosphate 5 kinase type I alpha antibody
- Phosphatidylinositol-4-phosphate 5-kinase type I alpha antibody
see all
Images
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All lanes : Anti-PIP5K1 alpha/PIP5K1A antibody (ab122049) at 1/250 dilution
Lane 1 : RT-4 cells lysate
Lane 2 : U-251 MG cells lysate
Lane 3 : Human plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate -
ab122049, at 1/125, staining PIP5K1 alpha/PIP5K1A in paraffin embedded Human tonsil tissue by Immunohistochemistry.
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ab122049, at 4 µg/ml, staining PIP5K1 alpha/PIP5K1A in Human cell line A-431 by Immunofluorescence.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab122049 has not yet been referenced specifically in any publications.