Key features and details
- Rabbit polyclonal to PIP5K1 alpha/PIP5K1A
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PIP5K1 alpha/PIP5K1A antibody
See all PIP5K1 alpha/PIP5K1A primary antibodies
DescriptionRabbit polyclonal to PIP5K1 alpha/PIP5K1A
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human PIP5K1 alpha/PIP5K1A aa 266-389.
Database link: Q99755
- Human tonsil tissue; RT-4 and U-251 MG cells lysate; Human cell line A-431
This product was previously labelled as PIP5K1 alpha
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionParticipates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. Mediates RAC1-dependent reorganization of actin filaments. Acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs. Contributes to the activation of PLD2.
Tissue specificityHighly expressed in heart, placenta, skeletal muscle, kidney and pancreas. Detected at lower levels in brain, lung and liver.
Sequence similaritiesContains 1 PIPK domain.
Cellular localizationCell membrane. Endomembrane system. Golgi apparatus > Golgi stack. Nucleus speckle. Associated with the plasma membrane and with internal membranes. Associated with Golgi stacks (By similarity). Detected on RAC1-induced plasma membrane ruffles, and on membrane ruffles induced by platelet-derived growth factor. Localizes to nuclear speckles and associates with TUT1 to regulate polyadenylation of selected mRNAs.
- Information by UniProt
- 68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha antibody
- Phosphatidylinositol 4 phosphate 5 kinase type I alpha antibody
- Phosphatidylinositol-4-phosphate 5-kinase type I alpha antibody
All lanes : Anti-PIP5K1 alpha/PIP5K1A antibody (ab122049) at 1/250 dilution
Lane 1 : RT-4 cells lysate
Lane 2 : U-251 MG cells lysate
Lane 3 : Human plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
ab122049, at 1/125, staining PIP5K1 alpha/PIP5K1A in paraffin embedded Human tonsil tissue by Immunohistochemistry.
ab122049, at 4 µg/ml, staining PIP5K1 alpha/PIP5K1A in Human cell line A-431 by Immunofluorescence.
ab122049 has not yet been referenced specifically in any publications.