Key features and details
- Rabbit polyclonal to PIP5K1 alpha/PIP5K1A
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PIP5K1 alpha/PIP5K1A antibody
See all PIP5K1 alpha/PIP5K1A primary antibodies
DescriptionRabbit polyclonal to PIP5K1 alpha/PIP5K1A
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Dog, Pig
Recombinant fragment corresponding to Human PIP5K1 alpha/PIP5K1A aa 1-180.
Database link: Q99755
- Recombinant human PIP5K1 beta protein (ab125547) can be used as a positive control in WB. 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates; CL1-0 xenograft tissue; A549 cells
This product was previously labelled as PIP5K1 alpha
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab137441 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 63 kDa.|
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionParticipates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. Mediates RAC1-dependent reorganization of actin filaments. Acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs. Contributes to the activation of PLD2.
Tissue specificityHighly expressed in heart, placenta, skeletal muscle, kidney and pancreas. Detected at lower levels in brain, lung and liver.
Sequence similaritiesContains 1 PIPK domain.
Cellular localizationCell membrane. Endomembrane system. Golgi apparatus > Golgi stack. Nucleus speckle. Associated with the plasma membrane and with internal membranes. Associated with Golgi stacks (By similarity). Detected on RAC1-induced plasma membrane ruffles, and on membrane ruffles induced by platelet-derived growth factor. Localizes to nuclear speckles and associates with TUT1 to regulate polyadenylation of selected mRNAs.
- Information by UniProt
- 68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha antibody
- Phosphatidylinositol 4 phosphate 5 kinase type I alpha antibody
- Phosphatidylinositol-4-phosphate 5-kinase type I alpha antibody
All lanes : Anti-PIP5K1 alpha/PIP5K1A antibody (ab137441) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 63 kDa
7.5% SDS PAGE
Immunohistochemical analysis of parafin-embedded CL1-0 xenograft tissue labelling PIP5K1 alpha/PIP5K1A with ab137441 at 1/100 dilution.
Immunofluorescence analysis of methanol-fixed A549 cells labelling PIP5K1 alpha/PIP5K1A with ab137441 at 1/500 dilution. The image in the lower panel is costained with a Hoechst 33342.
ab137441 has been referenced in 1 publication.
- Aoun J et al. Anoctamin 6 Contributes to Cl- Secretion in Accessory Cholera Enterotoxin (Ace)-stimulated Diarrhea: AN ESSENTIAL ROLE FOR PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE (PIP2) SIGNALING IN CHOLERA. J Biol Chem 291:26816-26836 (2016). PubMed: 27799301