Overview

  • Product name

    Anti-PIP5K1 alpha/PIP5K1A antibody
    See all PIP5K1 alpha/PIP5K1A primary antibodies
  • Description

    Rabbit polyclonal to PIP5K1 alpha/PIP5K1A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Dog, Pig
  • Immunogen

    Recombinant fragment corresponding to Human PIP5K1 alpha/PIP5K1A aa 1-180.
    Database link: Q99755

  • Positive control

    • Recombinant human PIP5K1 beta protein (ab125547) can be used as a positive control in WB. 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates; CL1-0 xenograft tissue; A549 cells
  • General notes

     This product was previously labelled as PIP5K1 alpha

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 63 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. Mediates RAC1-dependent reorganization of actin filaments. Acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs. Contributes to the activation of PLD2.
  • Tissue specificity

    Highly expressed in heart, placenta, skeletal muscle, kidney and pancreas. Detected at lower levels in brain, lung and liver.
  • Sequence similarities

    Contains 1 PIPK domain.
  • Cellular localization

    Cell membrane. Endomembrane system. Golgi apparatus > Golgi stack. Nucleus speckle. Associated with the plasma membrane and with internal membranes. Associated with Golgi stacks (By similarity). Detected on RAC1-induced plasma membrane ruffles, and on membrane ruffles induced by platelet-derived growth factor. Localizes to nuclear speckles and associates with TUT1 to regulate polyadenylation of selected mRNAs.
  • Information by UniProt
  • Database links

  • Alternative names

    • 68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha antibody
    • Phosphatidylinositol 4 phosphate 5 kinase type I alpha antibody
    • Phosphatidylinositol-4-phosphate 5-kinase type I alpha antibody
    • Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha antibody
    • PI4P5K Ia antibody
    • PI51A_HUMAN antibody
    • PIP5K A antibody
    • PIP5K1-alpha antibody
    • Pip5k1a antibody
    • PIP5KIalpha antibody
    • PtdIns(4)P-5-kinase 1 alpha antibody
    see all

Images

  • All lanes : Anti-PIP5K1 alpha/PIP5K1A antibody (ab137441) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate
    Lane 3 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 63 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of parafin-embedded CL1-0 xenograft tissue labelling PIP5K1 alpha/PIP5K1A with ab137441 at 1/100 dilution.

  • Immunofluorescence analysis of methanol-fixed A549 cells labelling PIP5K1 alpha/PIP5K1A with ab137441 at 1/500 dilution. The image in the lower panel is costained with a Hoechst 33342.

References

This product has been referenced in:

  • Aoun J  et al. Anoctamin 6 Contributes to Cl- Secretion in Accessory Cholera Enterotoxin (Ace)-stimulated Diarrhea: AN ESSENTIAL ROLE FOR PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE (PIP2) SIGNALING IN CHOLERA. J Biol Chem 291:26816-26836 (2016). Read more (PubMed: 27799301) »
See 1 Publication for this product

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