Overview

  • Product name
    Anti-PIP5K1C antibody [MAO-R1]
    See all PIP5K1C primary antibodies
  • Description
    Rabbit monoclonal [MAO-R1] to PIP5K1C
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Full length protein corresponding to Mouse PIP5K1C.

  • Positive control
    • K562, Human fetal kidney, C6, Neuro-2a, and 293T cell lysates
  • General notes

     

     

     This product was previously labelled as PIP5KI gamma

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    MAO-R1
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109192 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 80-90 kDa (predicted molecular weight: 73 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function
      Plays a role in membrane ruffling and assembly of clathrin-coated pits at the synapse. Mediates RAC1-dependent reorganization of actin filaments (By similarity). Participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate.
    • Involvement in disease
      Defects in PIP5K1C are the cause of lethal congenital contracture syndrome type 3 (LCCS3) [MIM:611369]; also known as multiple contractural syndrome Israeli Bedouin type B. LCCS is an autosomal recessive disorder characterized by early fetal hydrops and akinesia, the Pena-Shokeir phenotype, specific neuropathology with degeneration of anterior horn neurons and extreme skeletal muscle atrophy. LCCS3 patients present at birth with severe multiple joint contractures with severe muscle wasting and atrophy, mainly in the legs. LCCS3 can be distinguished from the original LCCS by the absence of hydrops, fractures, and multiple pterygia.
    • Sequence similarities
      Contains 1 PIPK domain.
    • Cellular localization
      Cell membrane. Cytoplasmic, associated with the plasma membrane. Detected in focal adhesion plaques, membrane ruffles and plasma membrane invaginations.
    • Information by UniProt
    • Database links
    • Alternative names
      • Diphosphoinositide kinase antibody
      • LCCS3 antibody
      • Phosphatidylinositol-4-phosphate 5-kinase type I gamma antibody
      • Phosphatidylinositol-4-phosphate 5-kinase type-1 gamma antibody
      • PI51C antibody
      • PI51C_HUMAN antibody
      • PIP5K GAMMA antibody
      • PIP5K1-gamma antibody
      • Pip5k1c antibody
      • PIP5KIgamma antibody
      • PtdIns(4)P 5 kinase gamma antibody
      • PtdIns(4)P-5-kinase 1 gamma antibody
      • Type I PIP kinase antibody
      see all

    Images

    • All lanes : Anti-PIP5K1C antibody [MAO-R1] (ab109192) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : Human fetal kidney cell lysate
      Lane 3 : C6 cell lysate
      Lane 4 : Neuro-2a cell lysate
      Lane 5 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 73 kDa

    References

    This product has been referenced in:
    • Durand N  et al. The phosphorylation status of PIP5K1C at serine 448 can be predictive for invasive ductal carcinoma of the breast. Oncotarget 9:36358-36370 (2018). Read more (PubMed: 30555634) »
    • Jafari N  et al. p70S6K1 (S6K1)-mediated Phosphorylation Regulates Phosphatidylinositol 4-Phosphate 5-Kinase Type I ? Degradation and Cell Invasion. J Biol Chem 291:25729-25741 (2016). Read more (PubMed: 27780861) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    Answer

    Thank you for contacting us and for your interest in ab109192. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase. If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information. Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates. To find out more about our Abreview system, please see the following link: https://www.abcam.com/abreviews I hope this information is helpful. Please do not hesitate to contact us again with any other questions.  

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up