Overview

  • Product name

    Anti-PIP5K3/PIKFYVE (phospho S307) antibody
    See all PIP5K3/PIKFYVE primary antibodies
  • Description

    Rabbit polyclonal to PIP5K3/PIKFYVE (phospho S307)
  • Host species

    Rabbit
  • Specificity

    ab62467 detects endogenous levels of PIP5K3/PIKFYVE only when phosphorylated at serine 307.

  • Tested applications

    Suitable for: ELISA, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human PIP5K3/PIKFYVE.

  • Positive control

    • Human brain tissue and HeLa cells.
  • General notes

     This product was previously labelled as PIP5K3

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab62467 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab62467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
ICC/IF 1/500 - 1/1000.
IHC-P 1/50 - 1/100.

Target

  • Function

    The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 3,5-bisphosphate. Required for endocytic-vacuolar pathway and nuclear migration. Plays a role in the biogenesis of endosome carrier vesicles (ECV)/ multivesicular bodies (MVB) transport intermediates from early endosomes.
  • Involvement in disease

    Corneal dystrophy, fleck
  • Sequence similarities

    Contains 1 DEP domain.
    Contains 1 FYVE-type zinc finger.
    Contains 1 PIPK domain.
  • Cellular localization

    Endosome membrane. Mainly associated with membranes of the late endocytic pathway.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1 phosphatidylinositol 3 phosphate 5 kinase antibody
    • 1 phosphatidylinositol 4 phosphate 5 kinase antibody
    • 1-phosphatidylinositol 3-phosphate 5-kinase antibody
    • CFD antibody
    • epididymis luminal protein 37 antibody
    • FAB1 antibody
    • FAB1, S. cerevisiae, homolog of antibody
    • FYV1_HUMAN antibody
    • FYVE finger containing phosphoinositide kinase antibody
    • FYVE finger-containing phosphoinositide kinase antibody
    • KIAA0981 antibody
    • MGC40423 antibody
    • p235 antibody
    • Phosphatidylinositol 3 phosphate 5 kinase type III antibody
    • Phosphatidylinositol 3 phosphate/phosphatidylinositol 5 kinase type III antibody
    • Phosphatidylinositol 3-phosphate 5-kinase antibody
    • Phosphatidylinositol 3-phosphate 5-kinase type III antibody
    • Phosphatidylinositol 4 phosphate 5 kinase type III antibody
    • Phosphoinositide kinase, FYVE finger containing antibody
    • PIKfyve antibody
    • PIP5K antibody
    • PIP5K3 antibody
    • PIPkin III antibody
    • PIPkin-III antibody
    • PtdIns(4)P 5 kinase antibody
    • Type III PIP kinase antibody
    • ZFYVE29 antibody
    • Zinc finger, FYVE domain containing 29 antibody
    see all

Images

  • ab62467, at 1/50 dilution, staining PIP5K3/PIKFYVE in paraffin embedded human brain tissue by Immunohistochemsitry in the absence (left image) or presence (right image) of the immunising peptide.

  • ab62467, at 1/500 dilution, staining PIP5K3/PIKFYVE in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.

References

ab62467 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (Mammary Epithelial)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
50 µg
Specification
Mammary Epithelial
Blocking step
Odyssey Blocking Buffer (Li-COR) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 30%

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Verified customer

Submitted Jul 08 2011

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