1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May act as a scaffold to localize and regulate type I PI(4)P 5-kinases to specific compartments within the cell, where they generate PI(4,5)P2 for actin nucleation, signaling and scaffold protein recruitment and conversion to PI(3,4,5)P3.
Contains 1 PIPK domain.
Cytoplasm. Membrane. Localized to large cytoplasmic vesicular structures.
Confocal immunofluorescent analysis of U-251MG cells labeling PIP5KL1 with ab170177 at 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclei (blue).
Western blot - Anti-PIP5KL1 antibody - N-terminal (ab170177)
Anti-PIP5KL1 antibody - N-terminal (ab170177) at 1/100 dilution + K562 cell lysate at 35 µg