Key features and details
- Rabbit polyclonal to Pirh2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Pirh2 antibody
See all Pirh2 primary antibodies
DescriptionRabbit polyclonal to Pirh2
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Pig, Rhesus monkey
Recombinant fragment within Human Pirh2 aa 59-261. The exact sequence is proprietary.
Database link: Q96PM5
- IHC-P: Human colon tissue. ICC/IF: HeLa cells. WB: NTERA-2 cl.D1 [NT2/D1] whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230551 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 30 kDa.|
|ICC/IF||1/100 - 1/1000.|
|IHC-P||Use a concentration of 5 µg/ml.|
FunctionMediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including p53/TP53, HDAC1 and CDKN1B. Preferentially acts on tetrameric p53/TP53. Contributes to the regulation of CDKN1B and p53/TP53 levels, and thereby contributes to the regulation of the cell cycle progression. Increases AR transcription factor activity.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 CHY-type zinc finger.
Contains 1 CTCHY-type zinc finger.
Contains 1 RING-type zinc finger.
modificationsSubject to ubiquitination and proteasomal degradation. Interaction with PLAGL2 or KAT5 enhances protein stability.
Cellular localizationNucleus. Nucleus speckle. Cytoplasm.
- Information by UniProt
- Androgen receptor N terminal interacting protein antibody
- Androgen receptor N-terminal-interacting protein antibody
- ARNIP antibody
Anti-Pirh2 antibody (ab230551) at 1/1000 dilution + NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 30 kDa
Formalin-fixed, paraffin-embedded human colon tissue stained for Pirh2 using ab230551 at 5 µg/ml in immunohistochemical analysis.
Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Pirh2 (green) using ab230551 at 1/500 dilution in ICC/IF.
Counter stained with Hoechst 33342 (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230551 has not yet been referenced specifically in any publications.