Overview

  • Product name

  • Description

    Rabbit polyclonal to Pirh2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Pirh2 aa 59-261. The exact sequence is proprietary.
    Database link: Q96PM5

  • Positive control

    • IHC-P: Human colon tissue. ICC/IF: HeLa cells. WB: NTERA-2 cl.D1 [NT2/D1] whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab230551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 30 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function

    Mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including p53/TP53, HDAC1 and CDKN1B. Preferentially acts on tetrameric p53/TP53. Contributes to the regulation of CDKN1B and p53/TP53 levels, and thereby contributes to the regulation of the cell cycle progression. Increases AR transcription factor activity.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 CHY-type zinc finger.
    Contains 1 CTCHY-type zinc finger.
    Contains 1 RING-type zinc finger.
  • Post-translational
    modifications

    Subject to ubiquitination and proteasomal degradation. Interaction with PLAGL2 or KAT5 enhances protein stability.
  • Cellular localization

    Nucleus. Nucleus speckle. Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Androgen receptor N terminal interacting protein antibody
    • Androgen receptor N-terminal-interacting protein antibody
    • ARNIP antibody
    • CH-rich-interacting match with PLAG1 antibody
    • CHIMP antibody
    • E3 ubiquitin-protein ligase Pirh2 antibody
    • hARNIP antibody
    • hPirh2 antibody
    • p53 induced protein with a RING H2 domain antibody
    • p53-induced RING-H2 protein antibody
    • PIRH2E antibody
    • PIRH2F antibody
    • PRO1996 antibody
    • RCHY1 antibody
    • Ring finger and CHY zinc finger domain containing 1 E3 ubiquitin protein ligase antibody
    • RING finger and CHY zinc finger domain-containing protein 1 antibody
    • RING finger protein 199 antibody
    • RNF199 antibody
    • ZCHY antibody
    • ZFP 363 antibody
    • zinc finger CHY type antibody
    • Zinc finger protein 363 antibody
    • ZN363_HUMAN antibody
    • ZNF363 antibody
    see all

Images

  • Anti-Pirh2 antibody (ab230551) at 1/1000 dilution + NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) whole cell lysate at 30 µg

    Predicted band size: 30 kDa

  • Formalin-fixed, paraffin-embedded human colon tissue stained for Pirh2 using ab230551 at 5 µg/ml in immunohistochemical analysis.

  • Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Pirh2 (green) using ab230551 at 1/500 dilution in ICC/IF.

    Counter stained with Hoechst 33342 (blue).

References

ab230551 has not yet been referenced specifically in any publications.

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