Recombinant Anti-Pirh2 antibody [EPR18553] - BSA and Azide free (ab251001)
![Western blot - Anti-Pirh2 antibody [EPR18553] - BSA and Azide free (ab251001)](https://www.abcam.com/ps/products/251/ab251001/Images/ab251001-389772-anti-pirh2-antibody-epr18553-western-blot-human-lysate.jpg "Western blot - Anti-Pirh2 antibody [EPR18553] - BSA and Azide free (ab251001)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18553] to Pirh2 - BSA and Azide free
- Suitable for: WB, IP, ICC/IF
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Pirh2 antibody [EPR18553] - BSA and Azide free
See all Pirh2 primary antibodies -
Description
Rabbit monoclonal [EPR18553] to Pirh2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, HepG2 and Daudi cell lysate.
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General notes
Ab251001 is the carrier-free version of ab189907. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab251001 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR18553 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab251001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
|
|
| IP |
Use at an assay dependent concentration.
|
|
| ICC/IF |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa). |
|
IP
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including p53/TP53, HDAC1 and CDKN1B. Preferentially acts on tetrameric p53/TP53. Contributes to the regulation of CDKN1B and p53/TP53 levels, and thereby contributes to the regulation of the cell cycle progression. Increases AR transcription factor activity. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 CHY-type zinc finger.
Contains 1 CTCHY-type zinc finger.
Contains 1 RING-type zinc finger. -
Post-translational
modificationsSubject to ubiquitination and proteasomal degradation. Interaction with PLAGL2 or KAT5 enhances protein stability. -
Cellular localization
Nucleus. Nucleus speckle. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 25898 Human
- Entrez Gene: 68098 Mouse
- Omim: 607680 Human
- SwissProt: Q96PM5 Human
- SwissProt: Q9CR50 Mouse
- Unigene: 48297 Human
- Unigene: 159453 Mouse
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Alternative names
- Androgen receptor N terminal interacting protein antibody
- Androgen receptor N-terminal-interacting protein antibody
- ARNIP antibody
see all
Images
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Western blot - Anti-Pirh2 antibody [EPR18553] - BSA and Azide free (ab251001)All lanes : Anti-Pirh2 antibody [EPR18553] (ab189907) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : RCHY1 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 30 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab189907).
Lanes 1-4: Merged signal (red and green). Green - ab189907 observed at 30 kDa. Red - loading control ab8245 observed at 37 kDa.
ab189907 Anti-Pirh2 antibody [EPR18553] was shown to specifically react with RCHY1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265478 (knockout cell lysate ab258171) was used. Wild-type and RCHY1 knockout samples were subjected to SDS-PAGE. ab189907 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Anti-Pirh2 antibody [EPR18553] - BSA and Azide free (ab251001)](https://www.abcam.com/ps/products/251/ab251001/Images/ab251001-2-benefits-of-recombinant-antibodies.png)
Anti-Pirh2 antibody [EPR18553] - BSA and Azide free (ab251001)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab251001 has not yet been referenced specifically in any publications.