For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    pirh2-antibody-epr18553-bsa-and-azide-free-ab251001.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ubiquitin
Share by email
Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Pirh2 antibody [EPR18553] - BSA and Azide free (ab251001)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-Pirh2 antibody [EPR18553] - BSA and Azide free (ab251001)
  • Anti-Pirh2 antibody [EPR18553] - BSA and Azide free (ab251001)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18553] to Pirh2 - BSA and Azide free
  • Suitable for: WB, IP, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Human

You may also be interested in

Conjugation
Product image
Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (ab236553)

View more associated products

Overview

  • Product name

    Anti-Pirh2 antibody [EPR18553] - BSA and Azide free
    See all Pirh2 primary antibodies
  • Description

    Rabbit monoclonal [EPR18553] to Pirh2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HepG2 and Daudi cell lysate.
  • General notes

    Ab251001 is the carrier-free version of ab189907. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab251001 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    EPR18553
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • p53 pathway

Associated products

  • Alternative Versions

    • Anti-Pirh2 antibody [EPR18553] (ab189907)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Conjugation kits

    • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • KO cell lines

    • Human RCHY1 (Pirh2) knockout HeLa cell line (ab265478)
  • KO cell lysates

    • Human RCHY1 (Pirh2) knockout HeLa cell lysate (ab258171)
  • Related Products

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab251001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.

Target

  • Function

    Mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including p53/TP53, HDAC1 and CDKN1B. Preferentially acts on tetrameric p53/TP53. Contributes to the regulation of CDKN1B and p53/TP53 levels, and thereby contributes to the regulation of the cell cycle progression. Increases AR transcription factor activity.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 CHY-type zinc finger.
    Contains 1 CTCHY-type zinc finger.
    Contains 1 RING-type zinc finger.
  • Post-translational
    modifications

    Subject to ubiquitination and proteasomal degradation. Interaction with PLAGL2 or KAT5 enhances protein stability.
  • Cellular localization

    Nucleus. Nucleus speckle. Cytoplasm.
  • Target information above from: UniProt accession Q96PM5 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 25898 Human
    • Entrez Gene: 68098 Mouse
    • Omim: 607680 Human
    • SwissProt: Q96PM5 Human
    • SwissProt: Q9CR50 Mouse
    • Unigene: 48297 Human
    • Unigene: 159453 Mouse
    • Alternative names

      • Androgen receptor N terminal interacting protein antibody
      • Androgen receptor N-terminal-interacting protein antibody
      • ARNIP antibody
      • CH-rich-interacting match with PLAG1 antibody
      • CHIMP antibody
      • E3 ubiquitin-protein ligase Pirh2 antibody
      • hARNIP antibody
      • hPirh2 antibody
      • p53 induced protein with a RING H2 domain antibody
      • p53-induced RING-H2 protein antibody
      • PIRH2E antibody
      • PIRH2F antibody
      • PRO1996 antibody
      • RCHY1 antibody
      • Ring finger and CHY zinc finger domain containing 1 E3 ubiquitin protein ligase antibody
      • RING finger and CHY zinc finger domain-containing protein 1 antibody
      • RING finger protein 199 antibody
      • RNF199 antibody
      • ZCHY antibody
      • ZFP 363 antibody
      • zinc finger CHY type antibody
      • Zinc finger protein 363 antibody
      • ZN363_HUMAN antibody
      • ZNF363 antibody
      see all

    Images

    • Western blot - Anti-Pirh2 antibody [EPR18553] - BSA and Azide free (ab251001)
      Western blot - Anti-Pirh2 antibody [EPR18553] - BSA and Azide free (ab251001)
      All lanes : Anti-Pirh2 antibody [EPR18553] (ab189907) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : RCHY1 knockout HeLa cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : Daudi cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 30 kDa



      This data was developed using the same antibody clone in a different buffer formulation (ab189907).

      Lanes 1-4: Merged signal (red and green). Green - ab189907 observed at 30 kDa. Red - loading control ab8245 observed at 37 kDa.

       ab189907 Anti-Pirh2 antibody [EPR18553] was shown to specifically react with RCHY1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265478 (knockout cell lysate ab258171) was used. Wild-type and RCHY1 knockout samples were subjected to SDS-PAGE. ab189907 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

       

       

    • Anti-Pirh2 antibody [EPR18553] - BSA and Azide free (ab251001)
      Anti-Pirh2 antibody [EPR18553] - BSA and Azide free (ab251001)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab251001? Please let us know so that we can cite the reference in this datasheet.

    ab251001 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab251001.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.