Overview

  • Product name
  • Description
    Goat polyclonal to Pirin / PIR
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human Pirin/ PIR (N terminal).
    Sequence:

    GSSKKVTLSVLSREC

  • Positive control
    • Human heart lysate. Lysate prepared from transfected HEK293 transiently expressing Pirin.
  • General notes

    Previously labelled as Pirin.

Properties

Applications

Our Abpromise guarantee covers the use of ab21202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 30-35 kDa (predicted molecular weight: 32 kDa).

Target

  • Function
    Possible transcriptional coregulator. May contribute to the regulation of cellular processes via its interaction with BCL3. May be required for efficient terminal myeloid maturation of hematopoietic cells. May play a role in the regulation of cell migration. May promote apoptosis when overexpressed. Has quercetin 2,3-dioxygenase activity (in vitro).
  • Tissue specificity
    Highly expressed in a subset of melanomas. Detected at very low levels in most tissues (at protein level). Expressed in all tissues, with highest level of expression in heart and liver.
  • Pathway
    Flavonoid metabolism; quercetin degradation.
  • Sequence similarities
    Belongs to the pirin family.
  • Cellular localization
    Nucleus. Cytoplasm. Predominantly localized in dot-like subnuclear structures. Cytoplasmic localization of PIR seems to positively correlate with melanoma progression.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3-dioxygenase PIR antibody
    • Iron binding nuclear protein antibody
    • Pir antibody
    • PIR_HUMAN antibody
    • Pirin antibody
    • Probable quercetin 2 antibody
    • probable quercetin 2,3-dioxygenase PIR antibody
    • Probable quercetinase antibody
    see all

Images

  • Anti-Pirin / PIR antibody (ab21202) at 0.3 µg/ml + Human Heart lysate (35µg protein in RIPA buffer).

    Developed using the ECL technique.

    Predicted band size: 32 kDa
    Observed band size: 30-35 kDa
    why is the actual band size different from the predicted?



    ab21202 (0.3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence.
  • Anti-Pirin / PIR antibody (ab21202) at 1 µg/ml + lysate prepared from transfected HEK293 cells transiently over-expressing Pirin / PIR.

    Predicted band size: 32 kDa
    Observed band size: 37 kDa why is the actual band size different from the predicted?
    Additional bands at: 32 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Brzóska K  et al. Basal PIR expression in HeLa cells is driven by NRF2 via evolutionary conserved antioxidant response element. Mol Cell Biochem 389:99-111 (2014). WB ; Human . Read more (PubMed: 24390086) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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