Overview

  • Product name

    Anti-Pirin/PIR antibody [2740C2]
    See all Pirin/PIR primary antibodies
  • Description

    Mouse monoclonal [2740C2] to Pirin/PIR
  • Host species

    Mouse
  • Tested applications

    Suitable for: Dot blot, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Pirin/PIR.

  • Positive control

    • HeLa whole cell lysate.
  • General notes

    Previously labelled as Pirin.

Properties

Applications

Our Abpromise guarantee covers the use of ab51360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent dilution.
WB 1/200. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).

Target

  • Function

    Possible transcriptional coregulator. May contribute to the regulation of cellular processes via its interaction with BCL3. May be required for efficient terminal myeloid maturation of hematopoietic cells. May play a role in the regulation of cell migration. May promote apoptosis when overexpressed. Has quercetin 2,3-dioxygenase activity (in vitro).
  • Tissue specificity

    Highly expressed in a subset of melanomas. Detected at very low levels in most tissues (at protein level). Expressed in all tissues, with highest level of expression in heart and liver.
  • Pathway

    Flavonoid metabolism; quercetin degradation.
  • Sequence similarities

    Belongs to the pirin family.
  • Cellular localization

    Nucleus. Cytoplasm. Predominantly localized in dot-like subnuclear structures. Cytoplasmic localization of PIR seems to positively correlate with melanoma progression.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3-dioxygenase PIR antibody
    • Iron binding nuclear protein antibody
    • Pir antibody
    • PIR_HUMAN antibody
    • Pirin antibody
    • Probable quercetin 2 antibody
    • probable quercetin 2,3-dioxygenase PIR antibody
    • Probable quercetinase antibody
    see all

Images

  • Anti-Pirin/PIR antibody [2740C2] (ab51360) at 1/200 dilution + HeLa whole cell lysate at 25 µg

    Secondary
    Anti mouse IgG antibody at 1/2500 dilution

    Predicted band size: 32 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?

References

ab51360 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab51360.
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