Overview

  • Product name

  • Description

    Rabbit polyclonal to Pirin/PIR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Pirin/PIR (internal sequence). The exact sequence is proprietary.
    Database link: O00625

  • Positive control

    • WB: Mouse liver tissue lysate; HeLa whole cell lysate. IHC-P: Mouse liver tissue; Rat brain stem tissue; MPG xenograft tissue. ICC/IF: HeLa cells.
  • General notes

    Previously labelled as Pirin.

Properties

Applications

Our Abpromise guarantee covers the use of ab227280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 32 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Possible transcriptional coregulator. May contribute to the regulation of cellular processes via its interaction with BCL3. May be required for efficient terminal myeloid maturation of hematopoietic cells. May play a role in the regulation of cell migration. May promote apoptosis when overexpressed. Has quercetin 2,3-dioxygenase activity (in vitro).
  • Tissue specificity

    Highly expressed in a subset of melanomas. Detected at very low levels in most tissues (at protein level). Expressed in all tissues, with highest level of expression in heart and liver.
  • Pathway

    Flavonoid metabolism; quercetin degradation.
  • Sequence similarities

    Belongs to the pirin family.
  • Cellular localization

    Nucleus. Cytoplasm. Predominantly localized in dot-like subnuclear structures. Cytoplasmic localization of PIR seems to positively correlate with melanoma progression.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3-dioxygenase PIR antibody
    • Iron binding nuclear protein antibody
    • Pir antibody
    • PIR_HUMAN antibody
    • Pirin antibody
    • Probable quercetin 2 antibody
    • probable quercetin 2,3-dioxygenase PIR antibody
    • Probable quercetinase antibody
    see all

Images

  • Paraffin-embedded MPG xenograft tissue stained for Pirin/PIR using ab227280  at 1/500 dilution in immunohistochemical analysis.

  • Anti-Pirin/PIR antibody (ab227280) at 1/1000 dilution + Mouse liver tissue lysate at 50 µg

    Predicted band size: 32 kDa



    12% SDS-PAGE gel.

  • Anti-Pirin/PIR antibody (ab227280) at 1000 cells + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

    Predicted band size: 32 kDa



    12% SDS-PAGE gel.

  • Paraffin-embedded mouse liver tissue stained for Pirin/PIR using ab227280 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded rat brain stem tissue stained for Pirin/PIR using ab227280 at 1/500 dilution in immunohistochemical analysis.

  • Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Pirin/PIR (green) using ab227280 at 1/500 dilution in ICC/IF. Nuclear Counterstain: Hoechst 33342 (blue).

References

ab227280 has not yet been referenced specifically in any publications.

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