Key features and details
- Mouse monoclonal [OTI4G5] to PISD
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-PISD antibody [OTI4G5]
See all PISD primary antibodies
DescriptionMouse monoclonal [OTI4G5] to PISD
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Chinese hamster, Orangutan
Recombinant fragment corresponding to Human PISD aa 80-340. Produced in E.coli (NP_055153).
VPTRLLSRAWGRLNQVELPHWLRRPVYSLYIWTFGVNMKEAAVEDLHHYR NLSEFFRRKLKPQARPVCGLHSVISPSDGRILNFGQVKNCEVEQVKGVTY SLESFLGPRMCTEDLPFPPAASCDSFKNQLVTREGNELYHCVIYLAPGDY HCFHSPTDWTVSHRRHFPGSLMSVNPGMARWIKELFCHNERVVLTGDWKH GFFSLTAVGATNVGSIRIYFDRDLHTNSPRHSKGSYNDFSFVTHTNREGV PMRKGEHLGEF
Database link: Q9UG56-2
- WB: pCMV6-ENTRY PISD transfected HEK-293T cell lysate. IHC-P: Human colon, lung and ovary adenocarcinoma tissues.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab236405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Perform heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 °C for 3 minutes.
|WB||1/2000. Predicted molecular weight: 47 kDa.|
PathwayPhospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from CDP-diacylglycerol: step 2/2.
Sequence similaritiesBelongs to the phosphatidylserine decarboxylase family.
- Information by UniProt
- DJ858B16 antibody
- dJ858B16.2 antibody
- DKFZp566G2246 antibody
All lanes : Anti-PISD antibody [OTI4G5] (ab236405) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY PISD transfected HEK-293T cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 47 kDa
Paraffin-embedded human colon tissue stained for PISD using ab236405 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human lung tissue stained for PISD using ab236405 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human ovary adenocarcinoma tissue stained for PISD using ab236405 at 1/150 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236405 has not yet been referenced specifically in any publications.