For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    pit1-antibody-2c11-ab10623.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling Transcription Domain Families Developmental Families POU
Share by email

Anti-Pit1 antibody [2C11] (ab10623)

  • Datasheet
Submit a review Q&A (1)References (3)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-Pit1 antibody [2C11] (ab10623)

    Key features and details

    • Mouse monoclonal [2C11] to Pit1
    • Suitable for: WB
    • Reacts with: Rat
    • Isotype: IgG1

    You may also be interested in

    Primary
    Product image
    Anti-gamma Catenin antibody [EPR17310] (ab184919)
    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    IHC
    Product image
    Mouse Specific HRP/AEC (ABC) Detection IHC kit (ab64258)

    View more associated products

    Overview

    • Product name

      Anti-Pit1 antibody [2C11]
      See all Pit1 primary antibodies
    • Description

      Mouse monoclonal [2C11] to Pit1
    • Host species

      Mouse
    • Tested Applications & Species

      Application Species
      WB
      Rat
      See all applications and species data
    • Immunogen

      Fusion protein:

      SAAECLPASN HATNVMSTAT GLHYSVPSCH YGNQPSTYGV MAGSLTPCLY KFPDHTLSHG FPPLHQPLLA EDPAASEFKQ ELRRKSKLVEE PIDMDSPEIR ELEQFANEFK VRRIKL

      , corresponding to amino acids 30-146 of Rat Pit1.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      Tissue culture media: 15% FBS, DMEN 4.5g/litre glucose and 10% NCTC 109.
      Additives: bovine insulin, oxaloacetic acid, glycine, L-glutamine and pen-strep.
    • Concentration information loading...
    • Purity

      Tissue culture supernatant
    • Clonality

      Monoclonal
    • Clone number

      2C11
    • Myeloma

      NS1
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Developmental Families
      • POU

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab10623 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Rat
    All applications
    Sheep
    Cow
    Human
    Pig
    Macaque monkey
    Application Abreviews Notes
    WB
    1/50. Detects a band of approximately 31 kDa (predicted molecular weight: 33 kDa).
    Notes
    WB
    1/50. Detects a band of approximately 31 kDa (predicted molecular weight: 33 kDa).

    Target

    • Function

      Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'.
    • Involvement in disease

      Defects in POU1F1 are the cause of pituitary hormone deficiency combined type 1 (CPHD1) [MIM:613038]. CPHD is characterized by impaired production of growth hormone (GH) and one or more of the other five anterior pituitary hormones.
    • Sequence similarities

      Belongs to the POU transcription factor family. Class-1 subfamily.
      Contains 1 homeobox DNA-binding domain.
      Contains 1 POU-specific domain.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession P28069 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 282315 Cow
      • Entrez Gene: 5449 Human
      • Entrez Gene: 25517 Rat
      • Entrez Gene: 397325 Sheep
      • Omim: 173110 Human
      • SwissProt: P28069 Human
      • SwissProt: Q04788 Pig
      • SwissProt: P10037 Rat
      • SwissProt: P79364 Sheep
      • Unigene: 591654 Human
      • Unigene: 10445 Rat
      see all
    • Alternative names

      • Dwarf antibody
      • GHF 1 antibody
      • GHF 1A antibody
      • GHF-1 antibody
      • GHF1 antibody
      • GHF1A antibody
      • Growth hormone factor 1 antibody
      • Hmp 1 antibody
      • Hmp1 antibody
      • Pit 1 antibody
      • Pit 1 beta antibody
      • PIT 1Z antibody
      • PiT-1 antibody
      • Pit1 beta antibody
      • PIT1_HUMAN antibody
      • PIT1Z antibody
      • Pituitary growth hormone antibody
      • Pituitary specific positive transcription factor 1 antibody
      • Pituitary-specific positive transcription factor 1 antibody
      • POU class 1 homeobox 1 antibody
      • POU domain class 1 transcription factor 1 antibody
      • POU domain transcriptional regulator antibody
      • POU1F1 antibody
      see all

    Images

    • Western blot - Anti-Pit1 antibody [2C11] (ab10623)
      Western blot - Anti-Pit1 antibody [2C11] (ab10623)

      At 1:40 dilution the western was very good on GH3 nuclear lysate which has an abundant expression of Pit1.

      At 1:40 dilution the western was very good on GH3 nuclear lysate which has an abundant expression of Pit1.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (3)

    Publishing research using ab10623? Please let us know so that we can cite the reference in this datasheet.

    ab10623 has been referenced in 3 publications.

    • Zhang Z  et al. MicroRNAs Regulate Pituitary Development, and MicroRNA 26b Specifically Targets Lymphoid Enhancer Factor 1 (Lef-1), Which Modulates Pituitary Transcription Factor 1 (Pit-1) Expression. J Biol Chem 285:34718-28 (2010). IHC-P ; Mouse . PubMed: 20807761
    • Sharp ZD  et al. Inactivating Pit-1 mutations alter subnuclear dynamics suggesting a protein misfolding and nuclear stress response. J Cell Biochem 92:664-78 (2004). PubMed: 15211565
    • Mancini MG  et al. Subnuclear partitioning and functional regulation of the Pit-1 transcription factor. J Cell Biochem 72:322-38 (1999). PubMed: 10022514

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    No staining on human formalin-fixed brain tissue. 1:50 dilution and tried low and high pH antigen retrieval.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 12 2012

    Answer

    Thank you very much for your call today and for letting us know about the trouble with this antibody.

    As we discussed, please keep me updated about the results after trying a different staining kit. I've looked through the literature on Google scholar but unfortunately could not find another reference using this antibody in IHC on FFPE tissues, so I don't have further information about the optimal protocol to use.

    I look forward to hearing from you. If you have any questions or need anything else from us, please let me know and I'll be happy to help.

    Read More

    Abcam Scientific Support

    Answered on Sep 12 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.