Anti-Pit1 antibody [2C11] (ab10623)
![Western blot - Anti-Pit1 antibody [2C11] (ab10623)](https://www.abcam.com/ps/products/10/ab10623/Images/ACF5F2.jpg "Western blot - Anti-Pit1 antibody [2C11] (ab10623)")
Key features and details
- Mouse monoclonal [2C11] to Pit1
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG1
Overview
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Product name
Anti-Pit1 antibody [2C11]
See all Pit1 primary antibodies -
Description
Mouse monoclonal [2C11] to Pit1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Rat
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Immunogen
Fusion protein:
SAAECLPASN HATNVMSTAT GLHYSVPSCH YGNQPSTYGV MAGSLTPCLY KFPDHTLSHG FPPLHQPLLA EDPAASEFKQ ELRRKSKLVEE PIDMDSPEIR ELEQFANEFK VRRIKL
, corresponding to amino acids 30-146 of Rat Pit1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Tissue culture media: 15% FBS, DMEN 4.5g/litre glucose and 10% NCTC 109.
Additives: bovine insulin, oxaloacetic acid, glycine, L-glutamine and pen-strep. -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
2C11 -
Myeloma
NS1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10623 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Rat
|
| All applications |
Sheep
Cow
Human
Pig
Macaque monkey
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/50. Detects a band of approximately 31 kDa (predicted molecular weight: 33 kDa).
|
| Notes |
|---|
|
WB
1/50. Detects a band of approximately 31 kDa (predicted molecular weight: 33 kDa). |
Target
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Function
Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'. -
Involvement in disease
Defects in POU1F1 are the cause of pituitary hormone deficiency combined type 1 (CPHD1) [MIM:613038]. CPHD is characterized by impaired production of growth hormone (GH) and one or more of the other five anterior pituitary hormones. -
Sequence similarities
Belongs to the POU transcription factor family. Class-1 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 282315 Cow
- Entrez Gene: 5449 Human
- Entrez Gene: 25517 Rat
- Entrez Gene: 397325 Sheep
- Omim: 173110 Human
- SwissProt: P28069 Human
- SwissProt: Q04788 Pig
- SwissProt: P10037 Rat
see all -
Alternative names
- Dwarf antibody
- GHF 1 antibody
- GHF 1A antibody
see all
Images
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Western blot - Anti-Pit1 antibody [2C11] (ab10623)
At 1:40 dilution the western was very good on GH3 nuclear lysate which has an abundant expression of Pit1.
At 1:40 dilution the western was very good on GH3 nuclear lysate which has an abundant expression of Pit1.
Datasheets and documents
References (3)
ab10623 has been referenced in 3 publications.
- Zhang Z et al. MicroRNAs Regulate Pituitary Development, and MicroRNA 26b Specifically Targets Lymphoid Enhancer Factor 1 (Lef-1), Which Modulates Pituitary Transcription Factor 1 (Pit-1) Expression. J Biol Chem 285:34718-28 (2010). IHC-P ; Mouse . PubMed: 20807761
- Sharp ZD et al. Inactivating Pit-1 mutations alter subnuclear dynamics suggesting a protein misfolding and nuclear stress response. J Cell Biochem 92:664-78 (2004). PubMed: 15211565
- Mancini MG et al. Subnuclear partitioning and functional regulation of the Pit-1 transcription factor. J Cell Biochem 72:322-38 (1999). PubMed: 10022514
