Anti-Pit1 antibody [5E4] (ab10545)

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Western blot - Anti-Pit1 antibody [5E4] (ab10545)
  • Immunocytochemistry/ Immunofluorescence - Anti-Pit1 antibody [5E4] (ab10545)
  • Immunoprecipitation - Anti-Pit1 antibody [5E4] (ab10545)

Key features and details

  • Mouse monoclonal [5E4] to Pit1
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Rat
  • Isotype: IgG2a

Overview

  • Product name

    Anti-Pit1 antibody [5E4]
    See all Pit1 primary antibodies

  • Description

    Mouse monoclonal [5E4] to Pit1

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    WB
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment:

    SAAECLPASN HATNVMSTAT GLHYSVPSCH YGNQPSTYGV MAGSLTPCLY KFPDHTLSHG FPPLHQPLLA EDPAASEFKQ ELRRKSKLVEE PIDMDSPEIR ELEQFANEFK VRRIKL

    , corresponding to amino acids 30-146 of Rat Pit1.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    Tissue culture media: 15% FBS, DMEN 4.5g/litre glucose and 10% NCTC 109.
    Additives: bovine insulin, oxaloacetic acid, glycine, L-glutamine and pen-strep.
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    5E4

  • Myeloma

    NS1

  • Isotype

    IgG2a

  • Light chain type

    kappa

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab10545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Rat
All applications
Sheep
Cow
Human
Pig
Macaque monkey
Application Abreviews Notes
WB
1/40. Detects a band of approximately 31 kDa (predicted molecular weight: 33 kDa).
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Notes
WB
1/40. Detects a band of approximately 31 kDa (predicted molecular weight: 33 kDa).
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.

Target

  • Function

    Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'.

  • Involvement in disease

    Defects in POU1F1 are the cause of pituitary hormone deficiency combined type 1 (CPHD1) [MIM:613038]. CPHD is characterized by impaired production of growth hormone (GH) and one or more of the other five anterior pituitary hormones.

  • Sequence similarities

    Belongs to the POU transcription factor family. Class-1 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Dwarf antibody
    • GHF 1 antibody
    • GHF 1A antibody
    • GHF-1 antibody
    • GHF1 antibody
    • GHF1A antibody
    • Growth hormone factor 1 antibody
    • Hmp 1 antibody
    • Hmp1 antibody
    • Pit 1 antibody
    • Pit 1 beta antibody
    • PIT 1Z antibody
    • PiT-1 antibody
    • Pit1 beta antibody
    • PIT1_HUMAN antibody
    • PIT1Z antibody
    • Pituitary growth hormone antibody
    • Pituitary specific positive transcription factor 1 antibody
    • Pituitary-specific positive transcription factor 1 antibody
    • POU class 1 homeobox 1 antibody
    • POU domain class 1 transcription factor 1 antibody
    • POU domain transcriptional regulator antibody
    • POU1F1 antibody
    see all

Images

  • Western blot - Anti-Pit1 antibody [5E4] (ab10545)
    Western blot - Anti-Pit1 antibody [5E4] (ab10545)

    At 1:40 dilution the western was very good on GH3 nuclear lysate which has an abundant expression of Pit1.

    At 1:40 dilution the western was very good on GH3 nuclear lysate which has an abundant expression of Pit1.
  • Immunocytochemistry/ Immunofluorescence - Anti-Pit1 antibody [5E4] (ab10545)
    Immunocytochemistry/ Immunofluorescence - Anti-Pit1 antibody [5E4] (ab10545)This image is courtesy of Zelton Dave Sharp
    IF using ab10545 carried out as per the reference (see below) by Zelton Dave Sharp of The University of Texas, San Antonio.
  • Immunoprecipitation - Anti-Pit1 antibody [5E4] (ab10545)
    Immunoprecipitation - Anti-Pit1 antibody [5E4] (ab10545)
    Antibody to Pit1 (ab10545) detects a 33 kDa band in IP followed by WB.

References (2)

Publishing research using ab10545? Please let us know so that we can cite the reference in this datasheet.

ab10545 has been referenced in 2 publications.

  • Choi SY  et al. Dipeptidyl peptidase-4 inhibitor gemigliptin protects against vascular calcification in an experimental chronic kidney disease and vascular smooth muscle cells. PLoS One 12:e0180393 (2017). WB ; Human . PubMed: 28686724
  • Mancini MG  et al. Subnuclear partitioning and functional regulation of the Pit-1 transcription factor. J Cell Biochem 72:322-38 (1999). PubMed: 10022514

Customer reviews and Q&As

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ChIP abreview for Anti-Pit1 antibody [5E4]

 Excellent
Abreviews
abreview image
Application
ChIP
Sample
Mouse Cell lysate - nuclear (Thyrotrope cell line (T-alpha-T-1))
Negative control
Empty region (called Ct2, Forward : TGCATAAATCAAATGCCCATA, Reverse : GCAAAGGTGATTTGCAGAAA), Beta-actin promoter region (Forward : CTTCCTTTGTCCCCTGAGCTT, Reverse : TCCATGGCGAACTATCAAGAC).
Specification
Thyrotrope cell line (T-alpha-T-1)
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 30 minute(s) and 0 second(s)
Specification of the cross-linking agent: Paraformaldehyde
Positive control
Thyroid stimulating hormone bêta sub-unit (Tshb) promoter region (Forward : CAGAGTTTCCAGGGAGAGGA, Reverse : CGAATTGCTGCTTTCTTATCTG). PIT1 is known to bind this Tshb promoter region in thyrotrope cells (Yumiko Kashiwabara, et al. 2009).
Read More

Mr. Vincent Pacini

Verified customer

Submitted Mar 26 2019

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