Anti-Pit1 antibody [5E4] (ab10545)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-Pit1 antibody [5E4]
See all Pit1 primary antibodies -
DescriptionMouse monoclonal [5E4] to Pit1
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Host speciesMouse
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Tested applicationsSuitable for: WB, ICC/IF, IPmore details
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Species reactivityReacts with: Mouse
Predicted to work with: Rat, Sheep, Cow, Human, Pig, Macaque monkey
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Immunogen
Recombinant fragment:
SAAECLPASN HATNVMSTAT GLHYSVPSCH YGNQPSTYGV MAGSLTPCLY KFPDHTLSHG FPPLHQPLLA EDPAASEFKQ ELRRKSKLVEE PIDMDSPEIR ELEQFANEFK VRRIKL
, corresponding to amino acids 30-146 of Rat Pit1.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferTissue culture media: 15% FBS, DMEN 4.5g/litre glucose and 10% NCTC 109.
Additives: bovine insulin, oxaloacetic acid, glycine, L-glutamine and pen-strep. -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone number5E4
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MyelomaNS1
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IsotypeIgG2a
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Light chain typekappa
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab10545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/40. Detects a band of approximately 31 kDa (predicted molecular weight: 33 kDa). | |
| ICC/IF | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. |
Target
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FunctionTranscription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'.
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Involvement in diseaseDefects in POU1F1 are the cause of pituitary hormone deficiency combined type 1 (CPHD1) [MIM:613038]. CPHD is characterized by impaired production of growth hormone (GH) and one or more of the other five anterior pituitary hormones.
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Sequence similaritiesBelongs to the POU transcription factor family. Class-1 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 282315 Cow
- Entrez Gene: 5449 Human
- Entrez Gene: 18736 Mouse
- Entrez Gene: 25517 Rat
- Entrez Gene: 397325 Sheep
- Omim: 173110 Human
- SwissProt: P28069 Human
- SwissProt: Q00286 Mouse
see all -
Alternative names
- Dwarf antibody
- GHF 1 antibody
- GHF 1A antibody
see all
Images
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![Western blot - Anti-Pit1 antibody [5E4] (ab10545)](https://www.abcam.com/ps/products/10/ab10545/Images/ACF5F2.jpg)
Western blot - Anti-Pit1 antibody [5E4] (ab10545)At 1:40 dilution the western was very good on GH3 nuclear lysate which has an abundant expression of Pit1.
At 1:40 dilution the western was very good on GH3 nuclear lysate which has an abundant expression of Pit1. -
![Immunocytochemistry/ Immunofluorescence - Anti-Pit1 antibody [5E4] (ab10545)](https://www.abcam.com/ps/products/10/ab10545/Images/ab10545.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Pit1 antibody [5E4] (ab10545)This image is courtesy of Zelton Dave SharpIF using ab10545 carried out as per the reference (see below) by Zelton Dave Sharp of The University of Texas, San Antonio. -
![Immunoprecipitation - Anti-Pit1 antibody [5E4] (ab10545)](https://www.abcam.com/ps/products/10/ab10545/Images/ab10545_21.jpg)
Immunoprecipitation - Anti-Pit1 antibody [5E4] (ab10545)Antibody to Pit1 (ab10545) detects a 33 kDa band in IP followed by WB.
Datasheets and documents
References
This product has been referenced in:
- Choi SY et al. Dipeptidyl peptidase-4 inhibitor gemigliptin protects against vascular calcification in an experimental chronic kidney disease and vascular smooth muscle cells. PLoS One 12:e0180393 (2017). WB ; Human . Read more (PubMed: 28686724) »
- Mancini MG et al. Subnuclear partitioning and functional regulation of the Pit-1 transcription factor. J Cell Biochem 72:322-38 (1999). Read more (PubMed: 10022514) »
