Anti-Pit1 antibody [5E4] (ab10545)
![Western blot - Anti-Pit1 antibody [5E4] (ab10545)](https://www.abcam.com/ps/products/10/ab10545/Images/ACF5F2.jpg "Western blot - Anti-Pit1 antibody [5E4] (ab10545)")
Key features and details
- Mouse monoclonal [5E4] to Pit1
- Suitable for: WB, ICC/IF, IP
- Reacts with: Rat
- Isotype: IgG2a
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Pit1 antibody [5E4]
See all Pit1 primary antibodies -
Description
Mouse monoclonal [5E4] to Pit1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Sheep, Cow, Human, Pig, Macaque monkey
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Immunogen
Recombinant fragment:
SAAECLPASN HATNVMSTAT GLHYSVPSCH YGNQPSTYGV MAGSLTPCLY KFPDHTLSHG FPPLHQPLLA EDPAASEFKQ ELRRKSKLVEE PIDMDSPEIR ELEQFANEFK VRRIKL
, corresponding to amino acids 30-146 of Rat Pit1. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Tissue culture media: 15% FBS, DMEN 4.5g/litre glucose and 10% NCTC 109.
Additives: bovine insulin, oxaloacetic acid, glycine, L-glutamine and pen-strep. -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
5E4 -
Myeloma
NS1 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/40. Detects a band of approximately 31 kDa (predicted molecular weight: 33 kDa).
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| ICC/IF |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
1/40. Detects a band of approximately 31 kDa (predicted molecular weight: 33 kDa). |
|
ICC/IF
Use at an assay dependent concentration. |
|
IP
Use at an assay dependent concentration. |
Target
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Function
Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'. -
Involvement in disease
Defects in POU1F1 are the cause of pituitary hormone deficiency combined type 1 (CPHD1) [MIM:613038]. CPHD is characterized by impaired production of growth hormone (GH) and one or more of the other five anterior pituitary hormones. -
Sequence similarities
Belongs to the POU transcription factor family. Class-1 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 282315 Cow
- Entrez Gene: 5449 Human
- Entrez Gene: 25517 Rat
- Entrez Gene: 397325 Sheep
- Omim: 173110 Human
- SwissProt: P28069 Human
- SwissProt: Q04788 Pig
- SwissProt: P10037 Rat
see all -
Alternative names
- Dwarf antibody
- GHF 1 antibody
- GHF 1A antibody
see all
Images
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Western blot - Anti-Pit1 antibody [5E4] (ab10545)
At 1:40 dilution the western was very good on GH3 nuclear lysate which has an abundant expression of Pit1.
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![Immunocytochemistry/ Immunofluorescence - Anti-Pit1 antibody [5E4] (ab10545)](https://www.abcam.com/ps/products/10/ab10545/Images/ab10545-18179-anti-pit1-antibody-5e4-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Pit1 antibody [5E4] (ab10545)This image is courtesy of Zelton Dave SharpIF using ab10545 carried out as per the reference (see below) by Zelton Dave Sharp of The University of Texas, San Antonio. -
![Immunoprecipitation - Anti-Pit1 antibody [5E4] (ab10545)](https://www.abcam.com/ps/products/10/ab10545/Images/ab10545-18178-anti-pit1-antibody-5e4-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Pit1 antibody [5E4] (ab10545)Antibody to Pit1 (ab10545) detects a 33 kDa band in IP followed by WB.
Two IgG monoclonal antibodies were used to immunoprecipitate pituitary-derived GH3 cellular proteins, and followed by Western analysis. Whole cell extracts (WCE) and immunoprecipitated fractions contain a prominent 33 kD band representing Pit-1. Nonspecific hybridoma supernatant was used as a negative control. Epitope mapping (data not shown) indicated that both IgG monoclonal antibodies recognize an epitope in the distal N-terminus within the ST activation domain of Pit-1
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab10545 has been referenced in 3 publications.
- Huang Y et al. IRF1-mediated downregulation of PGC1a contributes to cardiorenal syndrome type 4. Nat Commun 11:4664 (2020). PubMed: 32938919
- Choi SY et al. Dipeptidyl peptidase-4 inhibitor gemigliptin protects against vascular calcification in an experimental chronic kidney disease and vascular smooth muscle cells. PLoS One 12:e0180393 (2017). WB ; Human . PubMed: 28686724
- Mancini MG et al. Subnuclear partitioning and functional regulation of the Pit-1 transcription factor. J Cell Biochem 72:322-38 (1999). PubMed: 10022514
