Key features and details
- Rabbit polyclonal to Pit1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Pit1 antibody
See all Pit1 primary antibodies
DescriptionRabbit polyclonal to Pit1
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Pit1 aa 34-130.
Database link: P28069
- IHC-P: Human gastric cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab235915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/1000.|
FunctionTranscription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'.
Involvement in diseaseDefects in POU1F1 are the cause of pituitary hormone deficiency combined type 1 (CPHD1) [MIM:613038]. CPHD is characterized by impaired production of growth hormone (GH) and one or more of the other five anterior pituitary hormones.
Sequence similaritiesBelongs to the POU transcription factor family. Class-1 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.
- Information by UniProt
- Dwarf antibody
- GHF 1 antibody
- GHF 1A antibody
Paraffin-embedded human gastric cancer tissue stained for Pit1 with ab235915 at 1/600 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235915 has not yet been referenced specifically in any publications.