Overview

  • Product name

  • Description

    Rabbit polyclonal to Pit1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Pit1 aa 34-130.
    Database link: P28069

  • Positive control

    • IHC-P: Human gastric cancer tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab235915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000.

Target

  • Function

    Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'.
  • Involvement in disease

    Defects in POU1F1 are the cause of pituitary hormone deficiency combined type 1 (CPHD1) [MIM:613038]. CPHD is characterized by impaired production of growth hormone (GH) and one or more of the other five anterior pituitary hormones.
  • Sequence similarities

    Belongs to the POU transcription factor family. Class-1 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Dwarf antibody
    • GHF 1 antibody
    • GHF 1A antibody
    • GHF-1 antibody
    • GHF1 antibody
    • GHF1A antibody
    • Growth hormone factor 1 antibody
    • Hmp 1 antibody
    • Hmp1 antibody
    • Pit 1 antibody
    • Pit 1 beta antibody
    • PIT 1Z antibody
    • PiT-1 antibody
    • Pit1 beta antibody
    • PIT1_HUMAN antibody
    • PIT1Z antibody
    • Pituitary growth hormone antibody
    • Pituitary specific positive transcription factor 1 antibody
    • Pituitary-specific positive transcription factor 1 antibody
    • POU class 1 homeobox 1 antibody
    • POU domain class 1 transcription factor 1 antibody
    • POU domain transcriptional regulator antibody
    • POU1F1 antibody
    see all

Images

  • Paraffin-embedded human gastric cancer tissue stained for Pit1 with ab235915 at 1/600 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

References

ab235915 has not yet been referenced specifically in any publications.

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