Key features and details
- Rabbit polyclonal to Pit1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Pit1 antibody
See all Pit1 primary antibodies
DescriptionRabbit polyclonal to Pit1
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Cow, Dog, Turkey, Rhesus monkey
- IHC-P: Human pituitary tissue, tonsil tissue, pancreas tissue, liver tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab272639 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionTranscription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'.
Involvement in diseaseDefects in POU1F1 are the cause of pituitary hormone deficiency combined type 1 (CPHD1) [MIM:613038]. CPHD is characterized by impaired production of growth hormone (GH) and one or more of the other five anterior pituitary hormones.
Sequence similaritiesBelongs to the POU transcription factor family. Class-1 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.
- Information by UniProt
- Dwarf antibody
- GHF 1 antibody
- GHF 1A antibody
Paraffin-embedded formalin-fixed Human pituitary tissue stained for Pit1 using ab272639 at 1/2500 in immunohistochemical analysis.
Paraffin-embedded formalin-fixed Human tonsil tissue stained for Pit1 using ab272639 at 1/2500 in immunohistochemical analysis. Shows no expression as expected.
Paraffin-embedded formalin-fixed Human pancreas tissue stained for Pit1 using ab272639 at 1/2500 in immunohistochemical analysis. Shows no expression as expected.
Paraffin-embedded formalin-fixed Human liver tissue stained for Pit1 using ab272639 at 1/2500 in immunohistochemical analysis. Shows no expression as expected.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab272639 has not yet been referenced specifically in any publications.