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Phone call requesting more information on how the anti-PITPNC1 antibodies have been validated
Asked on Aug 24 2012
Thank you for contacting us and sorry for the delay in getting back to you. I have been trying to gather further information in relation the the three anti-PITPNC1 antibodies we have from our catalogue.
Taking each in turn:
Mouse polyclonal ab68013
This antibody shows a band of ˜ 32 kDa in Western blotting. This is to be expected as the blot was performed using PITPNC1 transfected 293T cell lysate. The transfected lysate was of the isoform 2 of PITPNC1 (SwissProt reference Q9UKF7) which is just under 32 kDa in size.
Rabbit polyclonal ab95225
This antibody has been used in western blotting on HL60 cell lysate. The band observed is in the region of 32 kDa. This could again be explained as the isoform 2 of PITPNC1. However, we have not performed any further experiments to confirm this and no siRNA experiments have been performed to asses if the protein levels diminish.
Rabbit polyclonal ab118213
This antibody shows a band of ˜38 kDa in Western blotting with a non-specific band at ˜70 kDa. The nature of this non-specific band has not been investigated. It may be that with further optimisation of the Western blotting protocol these may reduce.
Unfortunately we are not able to characterise all the antibodies we supply as fully as we would like. However, any information we do have we make every effort to share with the customer so that they are able to make an informed decision as to whether the antibodies are of interest to them or not.
As discussed over the phone, we are unfortunately unable to provide sample sizes of any or our products. This is due to the logistics that this would involve. However, we are happy to say that all of our products are covered by the Abpromise guarantee, so if you find that any antibody you purchase from us does not perform as stated on the datasheet and the problem is reported to us within 6 months of purchase you would be eligible for a replacement antibody or your money back. More information on the Abpromise can be found form the following link:
I hope this information has been of help. If you require any further information please do not hesitate to contact us again.
Answered on Aug 24 2012