Anti-PITRM1 antibody (ab111681)
Key features and details
- Rabbit polyclonal to PITRM1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PITRM1 antibody
See all PITRM1 primary antibodies -
Description
Rabbit polyclonal to PITRM1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-188 of Human PITRM1 (AAH01150).
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Positive control
- A549, HeLa, HepG2 and HCT116 cells and whole cell lysates; PC13 xenograft tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab111681 is purified by antigen-affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab111681 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 117 kDa. | |
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/500. |
Target
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Function
ATP-independent protease that degrades mitochondrial transit peptides after their cleavage. Also degrades other unstructured peptides. Specific for peptides in the range of 10 to 65 residues. Able to degrade amyloid beta A4 (APP) protein when it accumulates in mitochondrion, suggesting a link with Alzheimer disease. Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference. -
Tissue specificity
Widely expressed. Expressed at higher level in muscle and heart compared to brain, pancreas, liver, lung and placenta. -
Sequence similarities
Belongs to the peptidase M16 family. PreP subfamily. -
Post-translational
modificationsThe disulfide bond may lock the enzyme in a closed conformation under oxidized conditions, suggesting that it may participate in redox regulation of the enzyme. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 10531 Human
- Entrez Gene: 69617 Mouse
- SwissProt: Q5JRX3 Human
- SwissProt: Q8K411 Mouse
- Unigene: 528300 Human
- Unigene: 41933 Mouse
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Alternative names
- hMP1 antibody
- hPreP antibody
- KIAA1104 antibody
see all
Images
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Western blot - Anti-PITRM1 antibody (ab111681)Anti-PITRM1 antibody (ab111681) at 1/1000 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 117 kDa
5% SDS-PAGE. -
Immunocytochemistry/ Immunofluorescence - Anti-PITRM1 antibody (ab111681)ab111681 at 1/200 dilution staining PITRM1 in methanol-fixed HeLa cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITRM1 antibody (ab111681)ab111681 at 1/500 dilution staining PITRM1 in paraffin-embedded PC13 xenograft tissue by Immunohistochemistry.
Protocols
References (0)
ab111681 has not yet been referenced specifically in any publications.
