Anti-PITRM1 antibody (ab151421)
Key features and details
- Rabbit polyclonal to PITRM1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PITRM1 antibody
See all PITRM1 primary antibodies -
Description
Rabbit polyclonal to PITRM1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 145-534 of Human PITRM1 (UniProt ID: Q5JRX3).
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Positive control
- PC3 and U87 MG whole cell lysates; Human gastric ca tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 117 kDa.
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| IHC-P |
1/100 - 1/1000.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 117 kDa. |
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IHC-P
1/100 - 1/1000. |
Target
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Function
ATP-independent protease that degrades mitochondrial transit peptides after their cleavage. Also degrades other unstructured peptides. Specific for peptides in the range of 10 to 65 residues. Able to degrade amyloid beta A4 (APP) protein when it accumulates in mitochondrion, suggesting a link with Alzheimer disease. Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference. -
Tissue specificity
Widely expressed. Expressed at higher level in muscle and heart compared to brain, pancreas, liver, lung and placenta. -
Sequence similarities
Belongs to the peptidase M16 family. PreP subfamily. -
Post-translational
modificationsThe disulfide bond may lock the enzyme in a closed conformation under oxidized conditions, suggesting that it may participate in redox regulation of the enzyme. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 10531 Human
- Entrez Gene: 69617 Mouse
- Entrez Gene: 307081 Rat
- SwissProt: Q5JRX3 Human
- SwissProt: Q8K411 Mouse
- Unigene: 528300 Human
- Unigene: 41933 Mouse
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Alternative names
- hMP1 antibody
- hPreP antibody
- KIAA1104 antibody
see all
Images
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Western blot - Anti-PITRM1 antibody (ab151421)All lanes : Anti-PITRM1 antibody (ab151421) at 1/1000 dilution
Lane 1 : PC3 whole cell lysate
Lane 2 : U87 MG whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 117 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITRM1 antibody (ab151421)Immunohistochemical analysis of paraffin-embedded Human gastric ca tissue labeling PITRM1 with ab151421 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab151421 has not yet been referenced specifically in any publications.