Anti-PITRM1 antibody (ab241986)
Key features and details
- Rabbit polyclonal to PITRM1
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-PITRM1 antibody
See all PITRM1 primary antibodies -
Description
Rabbit polyclonal to PITRM1 -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human PITRM1 aa 987-1037. The exact sequence is proprietary.
Database link: Q5JRX3 -
Positive control
- IP: PITRM1 IP in HEK-293T whole cell lysate.
-
General notes
ab241986 has not performed satisfactorily when used for WB of PITRM1 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of PITRM1.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7-8 -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to PITRM1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab241986 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP |
Use at 2-10 µg/mg of lysate.
|
Notes |
---|
IP
Use at 2-10 µg/mg of lysate. |
Target
-
Function
ATP-independent protease that degrades mitochondrial transit peptides after their cleavage. Also degrades other unstructured peptides. Specific for peptides in the range of 10 to 65 residues. Able to degrade amyloid beta A4 (APP) protein when it accumulates in mitochondrion, suggesting a link with Alzheimer disease. Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference. -
Tissue specificity
Widely expressed. Expressed at higher level in muscle and heart compared to brain, pancreas, liver, lung and placenta. -
Sequence similarities
Belongs to the peptidase M16 family. PreP subfamily. -
Post-translational
modificationsThe disulfide bond may lock the enzyme in a closed conformation under oxidized conditions, suggesting that it may participate in redox regulation of the enzyme. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
-
Database links
- Entrez Gene: 10531 Human
- SwissProt: Q5JRX3 Human
- Unigene: 528300 Human
-
Alternative names
- hMP1 antibody
- hPreP antibody
- KIAA1104 antibody
see all
Images
-
PITRM1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab241986 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab241986 at 0.4 µg/ml.
Lane 1: ab241986 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG in HEK-293T whole cell lsyate.Detection: Chemiluminescence with an exposure time of 10 seconds
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab241986 has not yet been referenced specifically in any publications.