Key features and details
- Rabbit polyclonal to PITRM1
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PITRM1 antibody
See all PITRM1 primary antibodies
DescriptionRabbit polyclonal to PITRM1
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human PITRM1 aa 987-1037. The exact sequence is proprietary.
Database link: Q5JRX3
- IP: PITRM1 IP in HEK-293T whole cell lysate.
ab241986 has not performed satisfactorily when used for WB of PITRM1 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of PITRM1.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to PITRM1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab241986 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
FunctionATP-independent protease that degrades mitochondrial transit peptides after their cleavage. Also degrades other unstructured peptides. Specific for peptides in the range of 10 to 65 residues. Able to degrade amyloid beta A4 (APP) protein when it accumulates in mitochondrion, suggesting a link with Alzheimer disease. Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference.
Tissue specificityWidely expressed. Expressed at higher level in muscle and heart compared to brain, pancreas, liver, lung and placenta.
Sequence similaritiesBelongs to the peptidase M16 family. PreP subfamily.
modificationsThe disulfide bond may lock the enzyme in a closed conformation under oxidized conditions, suggesting that it may participate in redox regulation of the enzyme.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- hMP1 antibody
- hPreP antibody
- KIAA1104 antibody
PITRM1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab241986 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab241986 at 0.4 µg/ml.
Lane 1: ab241986 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG in HEK-293T whole cell lsyate.
Detection: Chemiluminescence with an exposure time of 10 seconds
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241986 has not yet been referenced specifically in any publications.