Overview

  • Product name

  • Description

    Rabbit polyclonal to PITRM1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PITRM1 aa 987-1037. The exact sequence is proprietary.
    Database link: Q5JRX3

  • Positive control

    • IP: PITRM1 IP in HEK-293T whole cell lysate.
  • General notes

    ab241986 has not performed satisfactorily when used for WB of PITRM1 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of PITRM1.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7-8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibody was affinity purified using an epitope specific to PITRM1 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab241986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      ATP-independent protease that degrades mitochondrial transit peptides after their cleavage. Also degrades other unstructured peptides. Specific for peptides in the range of 10 to 65 residues. Able to degrade amyloid beta A4 (APP) protein when it accumulates in mitochondrion, suggesting a link with Alzheimer disease. Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference.
    • Tissue specificity

      Widely expressed. Expressed at higher level in muscle and heart compared to brain, pancreas, liver, lung and placenta.
    • Sequence similarities

      Belongs to the peptidase M16 family. PreP subfamily.
    • Post-translational
      modifications

      The disulfide bond may lock the enzyme in a closed conformation under oxidized conditions, suggesting that it may participate in redox regulation of the enzyme.
    • Cellular localization

      Mitochondrion matrix.
    • Information by UniProt
    • Database links

    • Alternative names

      • hMP1 antibody
      • hPreP antibody
      • KIAA1104 antibody
      • Metalloprotease 1 antibody
      • MGC138192 antibody
      • MGC141929 antibody
      • mitochondrial antibody
      • MP1 antibody
      • Pitrilysin metallopeptidase 1 antibody
      • Pitrilysin metalloproteinase 1 antibody
      • PITRM 1 antibody
      • PITRM1 antibody
      • PreP antibody
      • PreP peptidasome antibody
      • PREP_HUMAN antibody
      • Presequence protease antibody
      • Presequence protease mitochondrial antibody
      see all

    Images

    • PITRM1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab241986 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab241986 at 0.4 µg/ml.

      Lane 1: ab241986 IP in HEK-293T whole cell lysate.
      Lane 2: Control IgG in HEK-293T whole cell lsyate.

      Detection: Chemiluminescence with an exposure time of 10 seconds

       

       

    References

    ab241986 has not yet been referenced specifically in any publications.

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