Key features and details
- Rabbit polyclonal to PITX1/BFT
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PITX1/BFT antibody
See all PITX1/BFT primary antibodies
DescriptionRabbit polyclonal to PITX1/BFT
Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- IHC-P: Human esophagus, skin, stomach and tonsil tissue. ICC/IF: U-2 OS cells.
This product was previously labelled as PITX1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab244308 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb.
Involvement in diseaseDefects in PITX1 are a cause of congenital clubfoot (CCF) [MIM:119800]; also known as talipes equinovarus (TEV). Clubfoot is a congenital limb deformity defined as fixation of the foot in cavus, adductus, varus, and equinus (i.e. inclined inwards, axially rotated outwards, and pointing downwards) with concomitant soft tissue abnormalities. Clubfoot may occur in isolation or as part of a syndrome. Clubfoot appears to be a multifactorial trait.
Sequence similaritiesBelongs to the paired homeobox family. Bicoid subfamily.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
- BFT antibody
- CCF antibody
- Hindlimb expressed homeobox protein backfoot antibody
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for PITX1/BFT (green) using ab244308 at 4 µg/ml in ICC/IF.
Formalin-fixed, paraffin-embedded human tonsil tissue stained for PITX1/BFT using ab244308 at 1/20 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human stomach tissue stained for PITX1/BFT using ab244308 at 1/20 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human skin tissue stained for PITX1/BFT using ab244308 at 1/20 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human esophagus tissue stained for PITX1/BFT using ab244308 at 1/20 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human pancreas tissue stained for PITX1/BFT using ab244308 at 1/20 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab244308 has not yet been referenced specifically in any publications.