• Product name

  • Description

    Rabbit polyclonal to PITX1/BFT
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Chimpanzee, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human PITX1/BFT (N terminal).
    Database link: NP_002644.3

  • Positive control

  • General notes

     This product was previously labelled as PITX1




Our Abpromise guarantee covers the use of ab70273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2500 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).
IP Use at 1-4 µg/mg of lysate.


  • Function

    May play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb.
  • Involvement in disease

    Defects in PITX1 are a cause of congenital clubfoot (CCF) [MIM:119800]; also known as talipes equinovarus (TEV). Clubfoot is a congenital limb deformity defined as fixation of the foot in cavus, adductus, varus, and equinus (i.e. inclined inwards, axially rotated outwards, and pointing downwards) with concomitant soft tissue abnormalities. Clubfoot may occur in isolation or as part of a syndrome. Clubfoot appears to be a multifactorial trait.
  • Sequence similarities

    Belongs to the paired homeobox family. Bicoid subfamily.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • BFT antibody
    • CCF antibody
    • Hindlimb expressed homeobox protein backfoot antibody
    • Hindlimb-expressed homeobox protein backfoot antibody
    • Homeobox protein PITX1 antibody
    • LBNBG antibody
    • Paired like homeodomain 1 antibody
    • Paired like homeodomain transcription factor 1 antibody
    • Paired-like homeodomain transcription factor 1 antibody
    • Pituitary homeo box 1 antibody
    • Pituitary homeobox 1 antibody
    • Pituitary otx related factor antibody
    • Pitx1 antibody
    • PITX1_HUMAN antibody
    • POTX antibody
    • PTX1 antibody
    see all


  • HeLa whole cell lysate (1 mg) was immunoprecipitated using ab70273 at 3 µg/mg lysate (lane 1) or an IgG control (lane 2). One quarter of the total IP reaction was loaded on to a gel and analysed by Western blot using ab70273 at 1 µg/ml. Bands were detected using chemiluminescence with an exposure time of 30 seconds.
  • All lanes : Anti-PITX1/BFT antibody (ab70273) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Predicted band size: 34 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?

    Exposure time: 3 minutes


This product has been referenced in:

  • Teng HW  et al. Protein tyrosine phosphatase 1B targets PITX1/p120RasGAP thus showing therapeutic potential in colorectal carcinoma. Sci Rep 6:35308 (2016). Read more (PubMed: 27752061) »
  • Pellicelli M  et al. E2F1 and TFDP1 Regulate PITX1 Expression in Normal and Osteoarthritic Articular Chondrocytes. PLoS One 11:e0165951 (2016). WB ; Human . Read more (PubMed: 27802335) »
See all 4 Publications for this product

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