Overview

  • Product name
  • Description
    Rabbit polyclonal to PITX2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: SVLAPGQPRS LDSSKHRLEV HTISDTSSPE AAEKDKSQQG KNEDVGAEDP, corresponding to a region within N terminal amino acids 37-86 of Human PITX2

  • Positive control
    • HepG2 whole cell lysate (ab7900). Human intestine.

Properties

Applications

Our Abpromise guarantee covers the use of ab32832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/62500.
WB Use a concentration of 2.5 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    May play an important role in development and maintenance of anterior structures. Isoform PTX2C is involved in left-right asymmetry the developing embryo.
  • Involvement in disease
    Defects in PITX2 are the cause of Axenfeld-Rieger syndrome type 1 (RIEG1) [MIM:180500]; also known as Rieger syndrome type 1. RIEG1 is an autosomal dominant defect characterized by hypodontia (partial anodontia), anal stenosis, hypertelorism, mental deficiency, agenesis of the facial bones, with malformation of the anterior chamber of the eye.
    Defects in PITX2 are the cause of iridogoniodysgenesis type 2 (IRID2) [MIM:137600]; also known as iridogoniodysgenesis syndrome 2 (IGDS2). It is an autosomal dominant inherited disease.
    Defects in PITX2 are a cause of Peters anomaly (PAN) [MIM:604229]. It is a congenital defect of the anterior chamber of the eye.
    Defects in PITX2 are associated with ring dermoid of cornea (RDC) [MIM:180550]. RDC is an autosomal dominantly inherited syndrome characterized by bilateral annular limbal dermoids with corneal and conjunctival extension.
  • Sequence similarities
    Belongs to the paired homeobox family. Bicoid subfamily.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • All1 responsive gene 1 antibody
    • ALL1 responsive protein ARP1 antibody
    • ALL1-responsive protein ARP1 antibody
    • ARP 1 antibody
    • ARP1 antibody
    • Brx 1 antibody
    • Brx1 antibody
    • Homeobox protein PITX2 antibody
    • IDG 2 antibody
    • IDG2 antibody
    • IGDS 2 antibody
    • IGDS antibody
    • IGDS2 antibody
    • IHG 2 antibody
    • IHG2 antibody
    • IRID 2 antibody
    • IRID2 antibody
    • MGC111022 antibody
    • MGC20144 antibody
    • Otlx 2 antibody
    • Otlx2 antibody
    • Paired like homeodomain transcription factor 2 antibody
    • Paired-like homeodomain transcription factor 2 antibody
    • Pituitary homeo box 2 antibody
    • Pituitary homeobox 2 antibody
    • PITX 2 antibody
    • pitx2 antibody
    • PITX2_HUMAN antibody
    • PTX 2 antibody
    • PTX2 antibody
    • RGS antibody
    • RIEG 1 antibody
    • RIEG antibody
    • Rieg bicoid related homeobox transcription factor 1 antibody
    • RIEG bicoid related homeobox transcription factor antibody
    • RIEG bicoid-related homeobox transcription factor antibody
    • RIEG1 antibody
    • RS antibody
    • Solurshin antibody
    see all

Images

  • Anti-PITX2 antibody (ab32832) at 2.5 µg/ml + HepG2 cell lysate

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size: 35 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling PITX2 with ab32832 at 4-8µg/ml. Cells with positive label (as indicated by the arrows) are epithelial cells of the intestinal villus.

References

ab32832 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Chicken Tissue sections (Neural tube)
Specification
Neural tube
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Dec 21 2011

Question
Answer

DISCOUNT CODE: *** Expiration date: January 31, 2012 I am pleased to hear you would like to test ab32832 in chicken tissue. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab32832 in chicken and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Skeletal muscle)
Loading amount
10 µg
Specification
Skeletal muscle
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C

Abcam user community

Verified customer

Submitted May 11 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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