Overview

  • Product name

    Anti-PITX2/RGS antibody [EPR22417]
    See all PITX2/RGS primary antibodies
  • Description

    Rabbit monoclonal [EPR22417] to PITX2/RGS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: IHC-Fr or IP
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant full length protein within Human PITX2/RGS aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q99697

  • Positive control

    • WB: Mouse and rat pituitary and skeletal muscle tissue lysate. IHC-P: Mouse and rat pituitary gland tissue.
  • General notes

     This product was previously labelled as PITX2

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab221142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 35 kDa.
  • Application notes
    Is unsuitable for IHC-Fr or IP.
  • Target

    • Function

      May play an important role in development and maintenance of anterior structures. Isoform PTX2C is involved in left-right asymmetry the developing embryo.
    • Involvement in disease

      Defects in PITX2 are the cause of Axenfeld-Rieger syndrome type 1 (RIEG1) [MIM:180500]; also known as Rieger syndrome type 1. RIEG1 is an autosomal dominant defect characterized by hypodontia (partial anodontia), anal stenosis, hypertelorism, mental deficiency, agenesis of the facial bones, with malformation of the anterior chamber of the eye.
      Defects in PITX2 are the cause of iridogoniodysgenesis type 2 (IRID2) [MIM:137600]; also known as iridogoniodysgenesis syndrome 2 (IGDS2). It is an autosomal dominant inherited disease.
      Defects in PITX2 are a cause of Peters anomaly (PAN) [MIM:604229]. It is a congenital defect of the anterior chamber of the eye.
      Defects in PITX2 are associated with ring dermoid of cornea (RDC) [MIM:180550]. RDC is an autosomal dominantly inherited syndrome characterized by bilateral annular limbal dermoids with corneal and conjunctival extension.
    • Sequence similarities

      Belongs to the paired homeobox family. Bicoid subfamily.
      Contains 1 homeobox DNA-binding domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • All1 responsive gene 1 antibody
      • ALL1 responsive protein ARP1 antibody
      • ALL1-responsive protein ARP1 antibody
      • ARP 1 antibody
      • ARP1 antibody
      • Brx 1 antibody
      • Brx1 antibody
      • Homeobox protein PITX2 antibody
      • IDG 2 antibody
      • IDG2 antibody
      • IGDS 2 antibody
      • IGDS antibody
      • IGDS2 antibody
      • IHG 2 antibody
      • IHG2 antibody
      • IRID 2 antibody
      • IRID2 antibody
      • MGC111022 antibody
      • MGC20144 antibody
      • Otlx 2 antibody
      • Otlx2 antibody
      • Paired like homeodomain transcription factor 2 antibody
      • Paired-like homeodomain transcription factor 2 antibody
      • Pituitary homeo box 2 antibody
      • Pituitary homeobox 2 antibody
      • PITX 2 antibody
      • pitx2 antibody
      • PITX2_HUMAN antibody
      • PTX 2 antibody
      • PTX2 antibody
      • RGS antibody
      • RIEG 1 antibody
      • RIEG antibody
      • Rieg bicoid related homeobox transcription factor 1 antibody
      • RIEG bicoid related homeobox transcription factor antibody
      • RIEG bicoid-related homeobox transcription factor antibody
      • RIEG1 antibody
      • RS antibody
      • Solurshin antibody
      see all

    Images

    • All lanes : Anti-PITX2/RGS antibody [EPR22417] (ab221142) at 1/1000 dilution

      Lane 1 : Mouse pituitary gland tissue
      Lane 2 : Rat pituitary gland tissue
      Lane 3 : Mouse skeletal Muscle tissue lysate
      Lane 4 : Rat skeletal muscle tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 35 kDa
      Observed band size: 30-38 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blovking/Diluting buffer and concentration: 5% NFDM/TBST

      These multiple bands observed in pituitary represent different PITX2/RGS isoforms (PITX2A, PITX2B, PITX2C, PITX2D).

    • Immunohistochemical analysis of paraffin-embedded mouse pituitary gland tissue labeling PITX2/RGS with ab221142 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Nuclear staining on mouse pituitary (PMID: 15761027, PMID: 16859901, PMID: 11807026) is observed. Counterstained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    • Immunohistochemical analysis of paraffin-embedded rat pituitary gland tissue labeling PITX2/RGS with ab221142 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Nuclear staining on rat pituitary (PMID: 15761027, PMID: 16859901, PMID: 11807026) is observed. Counterstained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    • Negative control.

      Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling PITX2/RGS with ab221142 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Negative control, no staining on rat liver (PMID: 11157981) is observed. Counterstained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    • Negative control.

      Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling PITX2/RGS with ab221142 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Negative control, no staining on mouse spleen (PMID: 11157981)  is observed. Counterstained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    References

    ab221142 has not yet been referenced specifically in any publications.

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