Overview

  • Product name

    Anti-PITX3/PTX3 antibody [EPR8868]
    See all PITX3/PTX3 primary antibodies
  • Description

    Rabbit monoclonal [EPR8868] to PITX3/PTX3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PITX3/PTX3 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • 293T, Y79, and U87-MG cell lysates. 293T cells
  • General notes

     

     

     This product was previously labelled as PITX3

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function

      Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. In addition to its importance during development, it also has roles in the long-term survival and maintenance of the mdDA neurons. Activates NR4A2/NURR1-mediated transcription of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. Acts by decreasing the interaction of NR4A2/NURR1 with the corepressor NCOR2/SMRT which acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state. Essential for the normal lens development and differentiation. Plays a critical role in the maintenance of mitotic activity of lens epithelial cells, fiber cell differentiation and in the control of the temporal and spatial activation of fiber cell-specific crystallins. Positively regulates FOXE3 expression and negatively regulates PROX1 in the anterior lens epithelium, preventing activation of CDKN1B/P27Kip1 and CDKN1C/P57Kip2 and thus maintains lens epithelial cells in cell cycle.
    • Tissue specificity

      Highly expressed in developing eye lens.
    • Involvement in disease

      Defects in PITX3 are a cause of cataract autosomal dominant (ADC) [MIM:604219]. Cataract is an opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. Cataract is the most common treatable cause of visual disability in childhood.
      Defects in PITX3 are a cause of anterior segment mesenchymal dysgenesis (ASMD) [MIM:107250]; also known as anterior segment ocular dysgenesis (ASOD). ASMD consists of a range of developmental defects in structures at the front of the eye, resulting from abnormal migration or differentiation of the neural crest derived mesenchymal cells that give rise to the cornea, iris, and other components of the anterior chamber during eye development. Mature anterior segment anomalies are associated with an increased risk of glaucoma and corneal opacity. Conditions falling within the phenotypic spectrum include aniridia, posterior embryotoxon, Axenfeld anomaly, Reiger anomaly/syndrome, Peters anomaly, and iridogoniodysgenesis.
      Defects in PITX3 are the cause of cataract posterior polar type 4 (CTPP4) [MIM:610623]. A subcapsular opacity, usually disk-shaped, located at the back of the lens. It can have a marked effect on visual acuity. Some patients affected by cataract posterior polar type 4 can present a severe phenotype including microphthalmia and neurological dysfunction.
    • Sequence similarities

      Belongs to the paired homeobox family. Bicoid subfamily.
      Contains 1 homeobox DNA-binding domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Homeobox protein PITX 3 antibody
      • Homeobox protein PITX3 antibody
      • MGC12766 antibody
      • Paired like homeodomain transcription factor 3 antibody
      • Paired-like homeodomain transcription factor 3 antibody
      • Pituitary homeobox 3 antibody
      • PITX 3 antibody
      • Pitx3 antibody
      • PITX3_HUMAN antibody
      • PTX 3 antibody
      • PTX3 antibody
      see all

    Images

    • All lanes : Anti-PITX3/PTX3 antibody [EPR8868] (ab134920) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Y79 cell lysate
      Lane 3 : U87-MG cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 32 kDa

    • Flow cytometric analysis of permeabilized 293T cells using ab134920 at 1/10 dilution (red) or a negative rabbit IgG (green).

    References

    ab134920 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Answer



    Concerning Anti-PITX3 antibody - Neuronal Marker (ab30734) and Anti-PITX3 antibody [EPR8868] (ab134920) I am happy to be able to offer you a blocking peptide for both of the antibodies: ab31696.

    https://www.abcam.com/index.html?datasheet=31696 (or use the following: https://www.abcam.com/index.html?datasheet=31696).

    I can confirm that the Human PITX3 peptide (ab31696) can be used as blocking peptide for both antibodies because they share the same immunogen.

    Read More

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