Key features and details
- Rabbit polyclonal to PIWIL1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-PIWIL1 antibody
See all PIWIL1 primary antibodies
DescriptionRabbit polyclonal to PIWIL1
SpecificityAt least three isoforms of PIWIL1 are known to exist; ab105393 will recognize the two longest isoforms. ab105393 is predicted to not cross-react with other PIWI family members.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human PIWIL1 aa 60-110 (N terminal).
- WB- HepG2 cell lysate IHC- Rat liver tissue
Storage instructionsShipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab105393 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 99 kDa.|
FunctionPlays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNA cleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability (By similarity). Isoform 3 may be a negative developmental regulator.
Tissue specificityDetected in most fetal and adult tissues. Expressed in testes, specifically in germline cells; detected in spermatocytes and spermatids during spermatogenesis. Increased expression in testicular tumors originating from embryonic germ cells with retention of germ cells phenotype. No expression in testicular tumors of somatic origin, such as Sertoli cell and Leydig cell tumors. Overexpressed in gastric cancer cells. Isoform 3 is ubiquitously expressed, and specifically in CD34+ hematopoietic progenitor cells but not in more differentiated cells.
Sequence similaritiesBelongs to the argonaute family. Piwi subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain.
DomainThe PAZ domain specifically recognizes binds the 2'-O-methylated 3'-end of piRNAs.
modificationsArginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein (TDRD1, TDRKH/TDRD2, RNF17/TDRD4, TDRD6, TDRD7 and TDRD9) and subsequent localization to the meiotic nuage, also named P granule.
Cellular localizationCytoplasm. Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. Also present in chromatoid body.
- Information by UniProt
- HIWI antibody
- MIWI antibody
- Piwi (Drosophila) like 1 antibody
Immunohistochemical analysis of rat liver tissue labeling PIWIL1 with ab105393 at 20 ug/mL.
All lanes : Anti-PIWIL1 antibody (ab105393) at 1 µg/ml
Lane 1 : HepG2 cell lysate without blocking peptide.
Lane 2 : HepG2 cell lysate with blocking peptide.
Lysates/proteins at 15 µg per lane.
Predicted band size: 99 kDa
Immunohistochemical analysis of rat liver tissue, staining PIWIL1 with ab105393 at 10 µg/mL.
ab105393 has been referenced in 2 publications.
- Sellitto A et al. Molecular and Functional Characterization of the Somatic PIWIL1/piRNA Pathway in Colorectal Cancer Cells. Cells 8:N/A (2019). PubMed: 31694219
- Chen Z et al. Stem cell protein Piwil1 endowed endometrial cancer cells with stem-like properties via inducing epithelial-mesenchymal transition. BMC Cancer 15:811 (2015). IHC . PubMed: 26506848