Key features and details
- Rabbit polyclonal to PIWIL2
- Suitable for: WB, ICC, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PIWIL2 antibody
See all PIWIL2 primary antibodies
DescriptionRabbit polyclonal to PIWIL2
SpecificityAt least three isoforms of PIWIL2 are known to exist; ab181340 will only detect the two longest isoforms. ab181340 is predicted to not cross-react with other PIWI protein family members.
Tested applicationsSuitable for: WB, ICC, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human PIWIL2 (internal sequence). The exact sequence is proprietary. Immunogen is 17 amino acids synthetic peptide within amino acids 410-460.
Database link: Q8TC59
- HepG2 cell lysate; HepG2 cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab181340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 110 kDa.|
|ICC||Use a concentration of 2.5 µg/ml.|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionPlays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate to similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates to a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors.
Tissue specificityExpressed in adult testis and in most tumors.
Sequence similaritiesBelongs to the argonaute family. Piwi subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain.
modificationsArginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein TDRD1 and subsequent localization to the meiotic nuage, also named P granule.
Cellular localizationCytoplasm. Present in chromatoid body. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis.
- Information by UniProt
- Cancer/testis antigen 80 antibody
- CT80 antibody
- Hiwi like antibody
ab181340 has been referenced in 7 publications.
- Tharp ME et al. Maximizing the ovarian reserve in mice by evading LINE-1 genotoxicity. Nat Commun 11:330 (2020). PubMed: 31949138
- Zhang D et al. Exosomes derived from Piwil2-induced cancer stem cells transform fibroblasts into cancer-associated fibroblasts. Oncol Rep 43:1125-1132 (2020). PubMed: 32323829
- Greeson KW et al. Detrimental effects of flame retardant, PBB153, exposure on sperm and future generations. Sci Rep 10:8567 (2020). PubMed: 32444626
- Li W et al. The Prognosis Value of PIWIL1 and PIWIL2 Expression in Pancreatic Cancer. J Clin Med 8:N/A (2019). PubMed: 31443431
- Gebert M et al. PIWI proteins contribute to apoptosis during the UPR in human airway epithelial cells. Sci Rep 8:16431 (2018). PubMed: 30401887
- Giebler M et al. Differential Regulation of PIWI-LIKE 2 Expression in Primordial Germ Cell Tumor Cell Lines by Promoter Methylation. Front Genet 9:375 (2018). WB ; Human . PubMed: 30294341
- Cao J et al. High expression of piwi-like RNA-mediated gene silencing 1 is associated with poor prognosis via regulating transforming growth factor-ß receptors and cyclin-dependent kinases in breast cancer. Mol Med Rep 13:2829-35 (2016). PubMed: 26847393