Anti-PIWIL2 antibody (ab36764)
Key features and details
- Rabbit polyclonal to PIWIL2
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
-
Product name
Anti-PIWIL2 antibody
See all PIWIL2 primary antibodies -
Description
Rabbit polyclonal to PIWIL2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
-
Immunogen
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab36764 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Mouse
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 110 kDa).
|
| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 110 kDa). |
Target
-
Function
Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate to similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates to a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors. -
Tissue specificity
Expressed in adult testis and in most tumors. -
Sequence similarities
Belongs to the argonaute family. Piwi subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
Post-translational
modificationsArginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein TDRD1 and subsequent localization to the meiotic nuage, also named P granule. -
Cellular localization
Cytoplasm. Present in chromatoid body. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. - Information by UniProt
-
Database links
- Entrez Gene: 57746 Mouse
- SwissProt: Q8CDG1 Mouse
- Unigene: 85253 Mouse
-
Alternative names
- Cancer/testis antigen 80 antibody
- CT80 antibody
- Hiwi like antibody
see all
Images
-
Western blot - Anti-PIWIL2 antibody (ab36764)Anti-PIWIL2 antibody (ab36764) at 1 µg/ml + Mouse Testis Whole Tissue Lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 110 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (15)
ab36764 has been referenced in 15 publications.
- Zhu Z et al. Rapamycin-mediated mTOR inhibition impairs silencing of sex chromosomes and the pachytene piRNA pathway in the mouse testis. Aging (Albany NY) 11:185-208 (2019). PubMed: 30636722
- Fu K et al. Biological and RNA regulatory function of MOV10 in mammalian germ cells. BMC Biol 17:39 (2019). PubMed: 31088452
- Bai S et al. Sox30 initiates transcription of haploid genes during late meiosis and spermiogenesis in mouse testes. Development 145:N/A (2018). PubMed: 29866902
- Jacobs DI et al. piRNA-8041 is downregulated in human glioblastoma and suppresses tumor growth in vitro and in vivo. Oncotarget 9:37616-37626 (2018). PubMed: 30701019
- Yoshimura T et al. Mouse GTSF1 is an essential factor for secondary piRNA biogenesis. EMBO Rep 19:N/A (2018). PubMed: 29437694