For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    piwil2-antibody-ab36764.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling DNA / RNA RNA Processing RNAi
Share by email

Anti-PIWIL2 antibody (ab36764)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (15)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-PIWIL2 antibody (ab36764)

    Key features and details

    • Rabbit polyclonal to PIWIL2
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

    You may also be interested in

    Primary
    Product image
    Anti-PIWIL1 antibody (ab12337)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Primary
    Product image
    Alexa Fluor® 488 Anti-SCP3 antibody [Cor 10G11/7] (ab205846)

    View more associated products

    Overview

    • Product name

      Anti-PIWIL2 antibody
      See all PIWIL2 primary antibodies
    • Description

      Rabbit polyclonal to PIWIL2
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Mouse
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Mouse PIWIL2 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
      (Peptide available as ab27821, ab27822)

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • RNA Processing
      • RNAi
      • Stem Cells
      • Germline Stem Cells
      • Embryonic Germ Cells
      • Epigenetics and Nuclear Signaling
      • RNAi
      • RISC
      • Epigenetics and Nuclear Signaling
      • RNAi
      • Argonaute and Piwi
      • Epigenetics and Nuclear Signaling
      • Nuclear Signaling Pathways
      • Nuclear Receptors
      • Nuclear Pore Complex

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab36764 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Mouse
    Application Abreviews Notes
    WB (1)
    Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 110 kDa).
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 110 kDa).

    Target

    • Function

      Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate to similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates to a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors.
    • Tissue specificity

      Expressed in adult testis and in most tumors.
    • Sequence similarities

      Belongs to the argonaute family. Piwi subfamily.
      Contains 1 PAZ domain.
      Contains 1 Piwi domain.
    • Post-translational
      modifications

      Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein TDRD1 and subsequent localization to the meiotic nuage, also named P granule.
    • Cellular localization

      Cytoplasm. Present in chromatoid body. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis.
    • Target information above from: UniProt accession Q8TC59 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 57746 Mouse
      • SwissProt: Q8CDG1 Mouse
      • Unigene: 85253 Mouse
      • Alternative names

        • Cancer/testis antigen 80 antibody
        • CT80 antibody
        • Hiwi like antibody
        • MILI antibody
        • Miwi like antibody
        • Piwi like 2 antibody
        • Piwi-like protein 2 antibody
        • Piwil2 antibody
        • PIWL2_HUMAN antibody
        see all

      Images

      • Western blot - Anti-PIWIL2 antibody (ab36764)
        Western blot - Anti-PIWIL2 antibody (ab36764)
        Anti-PIWIL2 antibody (ab36764) at 1 µg/ml + Mouse Testis Whole Tissue Lysate at 20 µg

        Secondary
        Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

        Performed under reducing conditions.

        Predicted band size: 110 kDa
        Observed band size: 100 kDa why is the actual band size different from the predicted?

      Protocols

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (15)

      Publishing research using ab36764? Please let us know so that we can cite the reference in this datasheet.

      ab36764 has been referenced in 15 publications.

      • Zhu Z  et al. Rapamycin-mediated mTOR inhibition impairs silencing of sex chromosomes and the pachytene piRNA pathway in the mouse testis. Aging (Albany NY) 11:185-208 (2019). PubMed: 30636722
      • Fu K  et al. Biological and RNA regulatory function of MOV10 in mammalian germ cells. BMC Biol 17:39 (2019). PubMed: 31088452
      • Bai S  et al. Sox30 initiates transcription of haploid genes during late meiosis and spermiogenesis in mouse testes. Development 145:N/A (2018). PubMed: 29866902
      • Jacobs DI  et al. piRNA-8041 is downregulated in human glioblastoma and suppresses tumor growth in vitro and in vivo. Oncotarget 9:37616-37626 (2018). PubMed: 30701019
      • Yoshimura T  et al. Mouse GTSF1 is an essential factor for secondary piRNA biogenesis. EMBO Rep 19:N/A (2018). PubMed: 29437694
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-2 of 2 Abreviews or Q&A

      Question

      Good morning,
      I would like to know the concentration of the antibody Anti-PIWIL2 (ab36764) that was used to produce the image reported in the TDS (https://www.abcam.com/PIWIL2-antibody-ab36764.html).
      Thank you very much for the consideration.

      Read More

      Abcam community

      Verified customer

      Asked on Feb 14 2012

      Answer

      Thank you for contacting us.

      The Western Blot image was obtained using 1 µg/ml of ab36764.

      The IHC-Fr image comes from a publication : http://www.ncbi.nlm.nih.gov/pubmed/20534472?dopt=Abstract. Unfortunately there is no information about the concentration used. I would suggest to contact the author. Otherwise I would recommendto use ab36764 in IHC-Fr ata concentration of 10 µg/ml as a starting pointand to optimise the results around this value.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on Feb 14 2012

      Western blot abreview for Anti-PIWIL2 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (Testis)
      Loading amount
      30 µg
      Specification
      Testis
      Gel Running Conditions
      Reduced Denaturing (10% gel)
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 04 2008

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.