- Datasheet
- Specific References (9)
- Protocols
Overview
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Product nameAnti-PIWIL2 antibody
See all PIWIL2 primary antibodies -
DescriptionRabbit polyclonal to PIWIL2
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-Fr, WB, Flow Cyt, ICC/IF, IP, Electron Microscopymore details
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Species reactivityReacts with: Mouse
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse MILI/ HILI.
(Peptide available as ab27821.)
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Positive control
- Mouse Testis Whole Tissue Lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab36764 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. PubMed: 20534472 | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 110 kDa).Can be blocked with Mouse PIWIL2 peptide (ab27821). | |
| Flow Cyt | Use at an assay dependent concentration. PubMed: 20460113 ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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| ICC/IF | Use at an assay dependent concentration. PubMed: 20460113 | |
| IP | Use at an assay dependent concentration. PubMed: 20460113 | |
| Electron Microscopy | Use at an assay dependent concentration. PubMed: 20011505 |
Target
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FunctionPlays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate to similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates to a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors.
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Tissue specificityExpressed in adult testis and in most tumors.
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Sequence similaritiesBelongs to the argonaute family. Piwi subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
Post-translational
modificationsArginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein TDRD1 and subsequent localization to the meiotic nuage, also named P granule. -
Cellular localizationCytoplasm. Present in chromatoid body. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis.
- Information by UniProt
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Database links
- Entrez Gene: 57746 Mouse
- SwissProt: Q8CDG1 Mouse
- Unigene: 85253 Mouse
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Alternative names
- Cancer/testis antigen 80 antibody
- CT80 antibody
- Hiwi like antibody
see all
Images
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Western blot - Anti-PIWIL2 antibody (ab36764)Anti-PIWIL2 antibody (ab36764) at 1 µg/ml + Mouse Testis Whole Tissue Lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 110 kDa
Observed band size: 100 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Frozen sections) - Anti-PIWIL2 antibody (ab36764)Image from Zheng K et al, Proc Natl Acad Sci U S A. 2010 Jun 29;107(26):11841-6. Epub 2010 Jun 1, Fig S5. DOI 10.1073/pnas.1003953107ab36764 staining PIWIL2 in murine testis tissue by Immunohistochemistry (Frozen sections). Chromatin was stained with DAPI. -
Immunocytochemistry/ Immunofluorescence - Anti-PIWIL2 antibody (ab36764)Zhou J et al. PLoS One., 9(2),. Fig 3. doi: 10.1371/journal.pone.0089471Testes section from adult mice were immunostained with anti-PIWIL2 (MILI) antibody ab36764. Nuclei were stained with DAPI. Testes were fixed in 4% PFA at 4°C for 3 hours and overnight respectively, dehydrated in 30% sucrose overnight, frozen in dry ice/95% ethanol, and sectioned at –20°C.
Protocols
References
This product has been referenced in:
- Feng D et al. Piwil2 is reactivated by HPV oncoproteins and initiates cell reprogramming via epigenetic regulation during cervical cancer tumorigenesis. Oncotarget 7:64575-64588 (2016). IP ; Human . Read more (PubMed: 27602489) »
- Zhou J et al. STK31/TDRD8, a germ cell-specific factor, is dispensable for reproduction in mice. PLoS One 9:e89471 (2014). IF ; Mouse . Read more (PubMed: 24586802) »
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