Overview

  • Product name

  • Description

    Rabbit polyclonal to PIWIL2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide:

    VITRYNNRTYRIDDVD

    , corresponding to internal sequence amino acids 418-433 of Human PIWIL2

  • Positive control

    • Testis tissue. Human fetal testis lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab85084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/500. Predicted molecular weight: 110 kDa.
IHC-P 1/100 - 1/400.

Target

  • Function

    Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate to similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates to a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors.
  • Tissue specificity

    Expressed in adult testis and in most tumors.
  • Sequence similarities

    Belongs to the argonaute family. Piwi subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • Post-translational
    modifications

    Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein TDRD1 and subsequent localization to the meiotic nuage, also named P granule.
  • Cellular localization

    Cytoplasm. Present in chromatoid body. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cancer/testis antigen 80 antibody
    • CT80 antibody
    • Hiwi like antibody
    • MILI antibody
    • Miwi like antibody
    • Piwi like 2 antibody
    • Piwi-like protein 2 antibody
    • Piwil2 antibody
    • PIWL2_HUMAN antibody
    see all

Images

  • Immunohistochemistry analysis of PIWIL2 in formalin-fixed paraffin-embedded human fetal testis tissue sections using ab85084 at 1/200 dilution.

  • Anti-PIWIL2 antibody (ab85084) at 1/200 dilution + Human fetal testis lysate

    Predicted band size: 110 kDa
    Observed band size: 110 kDa
    Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:

  • Feng D  et al. Piwil2 is reactivated by HPV oncoproteins and initiates cell reprogramming via epigenetic regulation during cervical cancer tumorigenesis. Oncotarget 7:64575-64588 (2016). WB, IHC ; Human . Read more (PubMed: 27602489) »
  • Berdasco M  et al. DNA Methylation Plasticity of Human Adipose-Derived Stem Cells in Lineage Commitment. Am J Pathol : (2012). WB . Read more (PubMed: 23031258) »
See all 2 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (CHO cells transiently overexpressing human PIWIL2)
Permeabilization
Yes - 0.1% Triton-X/PBS
Specification
CHO cells transiently overexpressing human PIWIL2
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 03 2017

Question
Answer

Thank you very much for your call today and for letting us know about the trouble with this antibody.

As we discussed, I'm sending a free of charge replacement on the order ***, which should arrive tomorrow.

Please keep me updated about the results with this replacement antibody, and if there is anything else that we can do for you don't hesitate to ask.

Have a nice day.

Read More

Question
Answer

Thank you for your email and confirming the details.

The protocol seems fine to me. However I have following questions;

- Did you try loading control? If yes, how was the results?
- Could you provide an image?
- In many cases no band problem can be overcome by incubating the antibodies over night or by decreasing the dilution. Could I suggest you to try the antibody overnight at 4C and 1/200 dilution if you haven't done so.

I hope these suggestions would be helpful. Please let me know if you have any question.

Read More

Answer

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.


I look forward to receiving your reply.

Read More

Answer

Thank you for contacting us.

The sequence homology between human and Platypus species seems quite OK for antibodies ab12337 and ab85084 cross reactivity. These antibodies may be tried for further tests. We can provide abrreview discount for these products.

Could you send us the required protein sequence of Echidna species for homology checks?

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up