For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
Lot number: GR41547-3
Inquiry: Good Morning, I have a problem with my Western Blotting: I used primary antibody Anti-piwil2 (ab 85084)but it seem does not identify positive control (mouse testis 30ug/ul). I incubate the membrane in blocking buffer (milk 5% in TBS with a minute percentage of detergent Tween 20),O.N, 4°C with gentle agitation on a shaker .After blocking wash the blot three times and added antibody Anti-piwil2 (dilute 1:500 with milk 5% in T-TBS O.N 4°C ). Could you give me suggestions to solve my problem?
Asked on Nov 05 2012
Thank you for contacting us.
I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.
I look forward to receiving your reply.
Answered on Nov 05 2012