Product nameAnti-PIWIL3 antibody
See all PIWIL3 primary antibodies
DescriptionMouse polyclonal to PIWIL3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Recombinant full length human PIWIL3 (NP_001008496, aa 1-882).
- PIWIL3 transfected 293T cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab77088 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).|
FunctionMay play a role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation.
Tissue specificityExpressed in testis.
Sequence similaritiesBelongs to the argonaute family. Piwi subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain.
Cellular localizationCytoplasm. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis.
- Information by UniProt
- HIWI3 antibody
- Piwi like 3 antibody
- Piwi-like protein 3 antibody
All lanes : Anti-PIWIL3 antibody (ab77088) at 5 µg/ml
Lane 1 : PIWIL3 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 97 kDa
Observed band size: 97 kDa
Additional bands at: 40 kDa, 60 kDa, 70 kDa. We are unsure as to the identity of these extra bands.