Anti-PIWIL3 antibody (ab93709)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-PIWIL3 antibody
See all PIWIL3 primary antibodies -
DescriptionRabbit polyclonal to PIWIL3
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide corresponding to Human PIWIL3 (internal sequence).
Database link: NM_001008496 -
Positive control
- Human testis tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MCF-7
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab93709 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | ||
| IHC-P |
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionMay play a role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation.
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Tissue specificityExpressed in testis.
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Sequence similaritiesBelongs to the argonaute family. Piwi subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
Cellular localizationCytoplasm. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis.
- Information by UniProt
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Database links
- Entrez Gene: 440822 Human
- Omim: 610314 Human
- SwissProt: Q7Z3Z3 Human
- Unigene: 448343 Human
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Alternative names
- HIWI3 antibody
- Piwi like 3 antibody
- Piwi-like protein 3 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-PIWIL3 antibody (ab93709)ICC/IF image of ab93709 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab93709 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIWIL3 antibody (ab93709)ab93709, at 1/100 dilution, staining PIWIL3 in formalin-fixed, paraffin-embedded Human testis tissue by Immunohistochemistry.
Datasheets and documents
References
This product has been referenced in:
- Cao J et al. High expression of piwi-like RNA-mediated gene silencing 1 is associated with poor prognosis via regulating transforming growth factor-ß receptors and cyclin-dependent kinases in breast cancer. Mol Med Rep 13:2829-35 (2016). Read more (PubMed: 26847393) »
