PIWIL3 overexpression 293T lysate (whole cell) (ab131153)

Overview

  • Product name

    PIWIL3 overexpression 293T lysate (whole cell)
  • Description

    PIWIL3 overexpression lysate
  • Tested applications

    Suitable for: WBmore details

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer

    Constituents: 0.01% Bromophenol blue, 2.34% Beta mercaptoethanol, 2% SDS, 0.61% Tris, 10% Glycerol
  • Concentration information loading...
  • Research areas

  • Background

    Function: May play a role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Similarity: Belongs to the argonaute family. Piwi subfamily. Contains 1 PAZ domain. Contains 1 Piwi domain. Tissue specificity: Expressed in testis.

Applications

Our Abpromise guarantee covers the use of ab131153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.

References

ab131153 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your enquiry and for your patience.

We have sourced andadded a new transfected 293T cell lysate to our catalog for you. I can recommend to review the online datasheet for further information:

ab131153 PIWIL3 293T Transfected Lysate
https://www.abcam.com/index.html?datasheet=131153 (or use the following: https://www.abcam.com/index.html?datasheet=131153).

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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