Anti-PIWIL4/PIWI antibody (ab111714)
Key features and details
- Rabbit polyclonal to PIWIL4/PIWI
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PIWIL4/PIWI antibody
See all PIWIL4/PIWI primary antibodies -
Description
Rabbit polyclonal to PIWIL4/PIWI -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Monkey -
Immunogen
Recombinant fragment corresponding to Human PIWIL4/PIWI aa 639-839. (AAH31060)
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Positive control
- MCF7 and IMR32 cells and whole cell lysates; 59T xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab111714 is purified by antigen-affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab111714 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 97 kDa.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer ( PH8.0)
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ICC/IF |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 97 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer ( PH8.0) |
ICC/IF
1/100 - 1/500. |
Target
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Function
Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association. The piRNA process acts upstream of known mediators of DNA methylation. Participates to a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity). May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci. -
Tissue specificity
Expressed in testis. According to PubMed:17544373, it is ubiquitously expressed. -
Sequence similarities
Belongs to the argonaute family. Piwi subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
Post-translational
modificationsArginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein (TDRD1, TDRKH/TDRD2 and TDRD9) and subsequent localization to the meiotic nuage, also named P granule. -
Cellular localization
Nucleus. Cytoplasm. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. PIWIL2/MILI is required for nuclear localization. - Information by UniProt
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Database links
- Entrez Gene: 143689 Human
- Omim: 610315 Human
- SwissProt: Q7Z3Z4 Human
- Unigene: 660188 Human
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Alternative names
- DKFZp686P01248 antibody
- FLJ36156 antibody
- HILI 2 antibody
see all
Images
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Anti-PIWIL4/PIWI antibody (ab111714) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg
Predicted band size: 97 kDa
7.5% SDS-PAGE. -
ab111714 at 1/200 dilution staining PIWIL4/PIWI in Paraformaldehyde-fixed MCF7 cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.
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ab111714 at 1/500 dilution staining PIWIL4/PIWI in Formalin-fixed, paraffin-embedded 59T xenograft tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab111714 has been referenced in 10 publications.
- Abdelhamid RF et al. piRNA/PIWI Protein Complex as a Potential Biomarker in Sporadic Amyotrophic Lateral Sclerosis. Mol Neurobiol 59:1693-1705 (2022). PubMed: 35015250
- Xie Q et al. piRNA-14633 promotes cervical cancer cell malignancy in a METTL14-dependent m6A RNA methylation manner. J Transl Med 20:51 (2022). PubMed: 35093098
- Liu J et al. piRNA-36741 regulates BMP2-mediated osteoblast differentiation via METTL3 controlled m6A modification. Aging (Albany NY) 13:23361-23375 (2021). PubMed: 34645714
- Singh G et al. Genome-wide profiling of the PIWI-interacting RNA-mRNA regulatory networks in epithelial ovarian cancers. PLoS One 13:e0190485 (2018). PubMed: 29320577
- Gebert M et al. PIWI proteins contribute to apoptosis during the UPR in human airway epithelial cells. Sci Rep 8:16431 (2018). PubMed: 30401887
- Jacobs DI et al. piRNA-8041 is downregulated in human glioblastoma and suppresses tumor growth in vitro and in vivo. Oncotarget 9:37616-37626 (2018). PubMed: 30701019
- Roy J & Mallick B Investigating piwi-interacting RNA regulome in human neuroblastoma. Genes Chromosomes Cancer 57:339-349 (2018). PubMed: 29516567
- Wang Z et al. The Role of PIWIL4, an Argonaute Family Protein, in Breast Cancer. J Biol Chem 291:10646-58 (2016). PubMed: 26957540
- Cordeiro A et al. PiwiRNA-651 as marker of treatment response and survival in classical Hodgkin lymphoma. Oncotarget 7:46002-46013 (2016). PubMed: 27329591
- Hashim A et al. RNA sequencing identifies specific PIWI-interacting small non-coding RNA expression patterns in breast cancer. Oncotarget 5:9901-10 (2014). PubMed: 25313140