Product nameAnti-PIWIL4/PIWI antibody
See all PIWIL4/PIWI primary antibodies
DescriptionRabbit polyclonal to PIWIL4/PIWI
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human PIWIL4/PIWI aa 546-838. The exact sequence is proprietary. (With tag; BC031060).
Database link: Q7Z3Z4
- HeLa, 231 and 293T cell lysates.
This product was previously labelled as PIWIL4
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Note: PBS without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab182760 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 97 kDa.|
FunctionPlays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association. The piRNA process acts upstream of known mediators of DNA methylation. Participates to a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity). May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci.
Tissue specificityExpressed in testis. According to PubMed:17544373, it is ubiquitously expressed.
Sequence similaritiesBelongs to the argonaute family. Piwi subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain.
modificationsArginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein (TDRD1, TDRKH/TDRD2 and TDRD9) and subsequent localization to the meiotic nuage, also named P granule.
Cellular localizationNucleus. Cytoplasm. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. PIWIL2/MILI is required for nuclear localization.
- Information by UniProt
- DKFZp686P01248 antibody
- FLJ36156 antibody
- HILI 2 antibody
All lanes : Anti-PIWIL4/PIWI antibody (ab182760) at 1/550 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 231 cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 40 µg per lane.
All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 97 kDa
Exposure time: 10 seconds
Gel: 8% SDS-PAGE
ab182760 has not yet been referenced specifically in any publications.