Overview

  • Product name

  • Description

    Rabbit polyclonal to PIWIL4/PIWI
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human PIWIL4/PIWI. (NP_689644.2)
    Database link: Q7Z3Z4

  • Positive control

    • Human Testis, Spermatogonia tissue.
  • General notes

     This product was previously labelled as PIWIL4

     

Properties

Applications

Our Abpromise guarantee covers the use of ab189356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/1000. Predicted molecular weight: 97 kDa.
IHC-P Use a concentration of 10 µg/ml.

Target

  • Function

    Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association. The piRNA process acts upstream of known mediators of DNA methylation. Participates to a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity). May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci.
  • Tissue specificity

    Expressed in testis. According to PubMed:17544373, it is ubiquitously expressed.
  • Sequence similarities

    Belongs to the argonaute family. Piwi subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • Post-translational
    modifications

    Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein (TDRD1, TDRKH/TDRD2 and TDRD9) and subsequent localization to the meiotic nuage, also named P granule.
  • Cellular localization

    Nucleus. Cytoplasm. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. PIWIL2/MILI is required for nuclear localization.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp686P01248 antibody
    • FLJ36156 antibody
    • HILI 2 antibody
    • HILI2 antibody
    • HIWI 2 antibody
    • HIWI2 antibody
    • Miwi 2 protein antibody
    • Miwi2 antibody
    • PIWI antibody
    • Piwi like 2 antibody
    • Piwi like 4 (Drosophila) antibody
    • Piwi like 4 antibody
    • Piwi like protein 4 antibody
    • PIWI like protein antibody
    • Piwi like RNA mediated gene silencing 4 antibody
    • Piwi-like protein 4 antibody
    • PIWIL 4 antibody
    • Piwil4 antibody
    • PIWL4_HUMAN antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Testis, Spermatogonia tissue labeling PIWIL4/PIWI with ab189356 at 10 µg/ml.

References

ab189356 has not yet been referenced specifically in any publications.

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