Anti-PIWIL4/PIWI antibody (ab21869)
Key features and details
- Rabbit polyclonal to PIWIL4/PIWI
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PIWIL4/PIWI antibody
See all PIWIL4/PIWI primary antibodies -
Description
Rabbit polyclonal to PIWIL4/PIWI -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Mouse PIWIL4/PIWI aa 800 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab23535) -
Positive control
- WB - Mouse testis tissue lysate, rat testis tissue lysate and human testis tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Coimmunogen
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab21869 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa). Block with 5% BSA for 1 hour and incubate antibody in TBST for 1 hour or more.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa). Block with 5% BSA for 1 hour and incubate antibody in TBST for 1 hour or more. |
Target
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Function
Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association. The piRNA process acts upstream of known mediators of DNA methylation. Participates to a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity). May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci. -
Tissue specificity
Expressed in testis. According to PubMed:17544373, it is ubiquitously expressed. -
Sequence similarities
Belongs to the argonaute family. Piwi subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
Post-translational
modificationsArginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein (TDRD1, TDRKH/TDRD2 and TDRD9) and subsequent localization to the meiotic nuage, also named P granule. -
Cellular localization
Nucleus. Cytoplasm. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. PIWIL2/MILI is required for nuclear localization. - Information by UniProt
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Database links
- Entrez Gene: 143689 Human
- Entrez Gene: 330890 Mouse
- Entrez Gene: 689972 Rat
- Omim: 610315 Human
- SwissProt: Q7Z3Z4 Human
- SwissProt: Q8CGT6 Mouse
- SwissProt: Q4G033 Rat
- Unigene: 660188 Human
see all -
Alternative names
- DKFZp686P01248 antibody
- FLJ36156 antibody
- HILI 2 antibody
see all
Images
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All lanes : Anti-PIWIL4/PIWI antibody (ab21869) at 1 µg/ml
Lane 1 : Mouse testis tissue lysate
Lane 2 : Human testis tissue lysate
Lane 3 : Rat testis tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 98 kDa
Observed band size: 98 kDa
Additional bands at: 56,52 kDa (possible isoform), 75 kDa (possible non-specific binding)
Exposure time: 12 minutesBlocking buffer: 2% BSA
Gel type: MOPS
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Lanes 1-2 : Anti-PIWIL4/PIWI antibody (ab21869) at 2 µg/ml
Lanes 3-4 : Anti-PIWIL4/PIWI antibody (ab21869) at 1 µg/ml
Lanes 1 & 3 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027)
Lanes 2 & 4 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027) withMouse PIWIL4/PIWI peptide (ab23535) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 98 kDa
Observed band size: 98 kDaab21869 specifically recognises PIWIL4/PIWI at 98kDa, which is demonstrated by the efficient blocking by the immunizing peptide (ab23535)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab21869 has been referenced in 6 publications.
- Chen G et al. PiRNA-63049 inhibits bone formation through Wnt/β-catenin signaling pathway. Int J Biol Sci 17:4409-4425 (2021). PubMed: 34803507
- Heng ZSL et al. The role of 17ß-estradiol-induced upregulation of Piwi-like 4 in modulating gene expression and motility in breast cancer cells. Oncol Rep 40:2525-2535 (2018). PubMed: 30226541
- Nishimura T et al. PNLDC1, mouse pre-piRNA Trimmer, is required for meiotic and post-meiotic male germ cell development. EMBO Rep 19:N/A (2018). Mouse . PubMed: 29444933
- Ding D et al. PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice. Nat Commun 8:819 (2017). IHC-P ; Mouse . PubMed: 29018194
- Keam SP et al. The human Piwi protein Hiwi2 associates with tRNA-derived piRNAs in somatic cells. Nucleic Acids Res 42:8984-95 (2014). WB, ICC/IF ; Human . PubMed: 25038252
- Yabuta Y et al. TDRD5 is required for retrotransposon silencing, chromatoid body assembly, and spermiogenesis in mice. J Cell Biol 192:781-95 (2011). IHC-Fr ; Mouse . PubMed: 21383078