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Trials 1-3 are my samples that I'm actually interested to see whether Piwil4 is expressed or not (so the answer is no). The positive mouse testis control I used was from Abcam, though unfortunately I don't know any further information regarding the lysate - it was from a former postdoc of our lab, aliquoted and only labeled as (mouse testis lysate/Abcam). I contacted him but he didn't remember whether its catalog number (obviously no lot number, either) was 4027 or 29289. I have to buy the lysate anyway, but just want to be sure what I'm purchasing is the right one - could you please check and give the lot number of ab29289 that is from fetal mice?
Also, thank you for the precise sequence of the immunogen for Ab21869. Don't know how the homology was analyzed, but to me, it shows even higher homology than I checked last time with a broader region (aa.800-end). Please check out the attached file.
Besides that, I also had a chance to try the antibody (21869) from one of my colleagues - unfortunately the result was pretty much the same - high background, and not detecting overexpressed-Piwil4. However, the person from whom I borrowed this 21869 antibody said that his previous batch was much better than this one (though he didn't do WB with these antibodies).
So here is the bottom line. I'd like to have Ab87939 with a different lot number that I've been using. For Ab21869, I am willing to buy it myself, but want to try the lot number 15736 if possible. Can I order it in that way? Lastly, the same for the mouse testis lysate (ab29289) - please let me know whether there is any lysate lot from fetal mice. I'd like to buy the real 'positive' control.
Thank you for your help,
Asked on Nov 20 2012
Thank you very much for your reply.
We have two lots of ab29289 in stock, so I'll check with the lab whether either of these is from fetal mice. I will get back to you as soon as I have this information.
I'll be happy to send a new lot of ab87939. Could you provide me with your original order or PO number, and I'll send this out promptly?
Unfortunately we only have the same lot of ab21869 in stock.
I look forward to hearing from you. If you have further questions or need anything else, please don't hesitate to ask.
Answered on Nov 20 2012