• Product name

    Anti-PKA alpha + beta (catalytic subunits) (phospho T197) antibody
    See all PKA alpha + beta (catalytic subunits) primary antibodies
  • Description

    Rabbit polyclonal to PKA alpha + beta (catalytic subunits) (phospho T197)
  • Host species

  • Specificity

    ab59218 detects endogenous levels of PKA alpha + beta (catalytic subunit) only when phosphorylated at threonine 197.
  • Tested applications

    Suitable for: ELISA, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic phosphopeptide derived from human PKA alpha + beta (catalytic subunit)around the phosphorylation site of threonine 197 (T-W-TP-L-C).

  • Positive control

    • Human colon carcinoma tissue and mouse brain cell extracts.



Our Abpromise guarantee covers the use of ab59218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).


  • Relevance

    PRKACA and PRKACB are members of the Ser/Thr protein kinase family and are a catalytic subunit of cAMP-dependent protein kinase. cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits.
  • Cellular localization

    Cytoplasm. Nucleus. Note=Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm
  • Database links

  • Alternative names

    • cAMP dependent protein kinase beta catalytic subunit antibody
    • cAMP dependent protein kinase alpha catalytic subunit antibody
    • cAMP dependent protein kinase catalytic subunit alpha antibody
    • cAMP dependent protein kinase catalytic subunit beta antibody
    • PKA C alpha antibody
    • PKA C beta antibody
    • PKACA antibody
    • PKACB antibody
    • PRKACA antibody
    • PRKACB antibody
    • Protein kinase cAMP dependent catalytic alpha antibody
    • Protein kinase cAMP dependent catalytic beta antibody
    see all


  • ab59218 at 1/50 dilution staining PKA alpha + beta (catalytic subunit) in human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence (left) or presence (right) of the immunising peptide.
  • All lanes : Anti-PKA alpha + beta (catalytic subunits) (phospho T197) antibody (ab59218) at 1/500 dilution

    Lane 1 : mouse brain cell extract
    Lane 2 : mouse brain cell extract with immunising peptide

    Predicted band size: 41 kDa
    Observed band size: 41 kDa


This product has been referenced in:

  • Skubal M  et al. Altered splicing leads to reduced activation of CPEB3 in high-grade gliomas. Oncotarget 7:41898-41912 (2016). IHC . Read more (PubMed: 27256982) »
See 1 Publication for this product

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