Product nameAnti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] - BSA and Azide free
See all PKA alpha/beta/gamma (catalytic subunit) primary antibodies
DescriptionRabbit monoclonal [EP2606Y] to PKA alpha/beta/gamma (catalytic subunit) (phospho T197) - BSA and Azide free
Tested applicationsSuitable for: WB, IP, IHC-P, ICC, Dot blotmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human PKA alpha/beta/gamma (catalytic subunit) aa 150-250 (phospho T197). The exact sequence is proprietary.
- WB: HeLa cell lysate treated with calyculin A.
Ab227848 is the carrier-free version of ab75991. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Concentration information loading...
Our Abpromise guarantee covers the use of ab227848 in the following tested applications.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Use of HRP-conjugated or polymerized HRP secondary antibodies recommended, stronger signals have been found using the polymerized HRP secondary.
|ICC||Use at an assay dependent concentration.|
|Dot blot||Use at an assay dependent concentration.|
- cAMP dependent protein kinase catalytic subunit alpha antibody
- cAMP dependent protein kinase catalytic subunit beta antibody
- cAMP dependent protein kinase catalytic subunit gamma antibody
Dot blot analysis of various peptides labelling PKA gamma (catalytic subunit) (phospho T197) with ab75991 at a dilution of 1/1000. ab97051, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/100000).
Lane 1 - PKA alpha/beta/gamma (catalytic subunit) (pT197) phospho peptide.
Lane 2 - PKA alpha/beta/gamma (catalytic subunit) non-phospho peptide.
Lane 3 - PRKX/Y (pT203) phospho peptide.
Lane 4 - PRKX/Y non-phospho peptide.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75991).
This IHC data was generated using the same anti- phospho PKA alpha/beta/gamma antibody clone, EP2606Y, in a different buffer formulation (cat# ab75991).
ab75991, at a 1/100 dilution, staining PKA gamma (catalytic subunit) in formalin fixed, paraffin embedded human testis tissue by Immunohistochemistry.
This product has been referenced in:
- Li J et al. Linarin promotes osteogenic differentiation by activating the BMP-2/RUNX2 pathway via protein kinase A signaling. Int J Mol Med 37:901-10 (2016). Read more (PubMed: 26935542) »
- Ferdaus MZ et al. SPAK and OSR1 play essential roles in potassium homeostasis through actions on the distal convoluted tubule. J Physiol 594:4945-66 (2016). Read more (PubMed: 27068441) »