Recombinant
RabMAb

Recombinant Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] - BSA and Azide free (ab227848)

Overview

  • Product name

    Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] - BSA and Azide free
    See all PKA alpha/beta/gamma (catalytic subunit) primary antibodies
  • Description

    Rabbit monoclonal [EP2606Y] to PKA alpha/beta/gamma (catalytic subunit) (phospho T197) - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC, Dot blotmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PKA alpha/beta/gamma (catalytic subunit) aa 150-250 (phospho T197). The exact sequence is proprietary.

  • Positive control

    • WB: HeLa cell lysate treated with calyculin A.
  • General notes

    Ab227848 is the carrier-free version of ab75991. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EP2606Y
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab227848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Use of HRP-conjugated or polymerized HRP secondary antibodies recommended, stronger signals have been found using the polymerized HRP secondary.

ICC Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    Images

    • Dot blot analysis of various peptides labelling PKA gamma (catalytic subunit) (phospho T197) with ab75991 at a dilution of 1/1000. ab97051, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/100000).

      Lane 1 - PKA alpha/beta/gamma (catalytic subunit) (pT197) phospho peptide.

      Lane 2 - PKA alpha/beta/gamma (catalytic subunit) non-phospho peptide.

      Lane 3 - PRKX/Y (pT203) phospho peptide.

      Lane 4 - PRKX/Y non-phospho peptide.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75991).

    • This IHC data was generated using the same anti- phospho PKA alpha/beta/gamma antibody clone, EP2606Y, in a different buffer formulation (cat# ab75991).

      ab75991, at a 1/100 dilution, staining PKA gamma (catalytic subunit) in formalin fixed, paraffin embedded human testis tissue by Immunohistochemistry.

    References

    This product has been referenced in:

    • Li J  et al. Linarin promotes osteogenic differentiation by activating the BMP-2/RUNX2 pathway via protein kinase A signaling. Int J Mol Med 37:901-10 (2016). Read more (PubMed: 26935542) »
    • Ferdaus MZ  et al. SPAK and OSR1 play essential roles in potassium homeostasis through actions on the distal convoluted tubule. J Physiol 594:4945-66 (2016). Read more (PubMed: 27068441) »
    See all 6 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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