Overview

  • Product name

    Anti-PKA beta (catalytic subunit) antibody
    See all PKA beta (catalytic subunit) primary antibodies
  • Description

    Rabbit polyclonal to PKA beta (catalytic subunit)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant full length protein corresponding to Human PKA beta (catalytic subunit).
    Database link: P22694

  • Positive control

    • SW480 cell lysate and U2OS cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab197047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 41 kDa.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux.
  • Tissue specificity

    Isoform 1 is most abundant in the brain, with low level expression in kidney. Isoform 2 is predominantly expressed in thymus, spleen and kidney. Isoform 3 and isoform 4 are only expressed in the brain.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively.
  • Cellular localization

    Cytoplasm. Nucleus. Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • cAMP-dependent protein kinase catalytic beta subunit isoform 4ab antibody
    • cAMP-dependent protein kinase catalytic subunit beta antibody
    • KAPCB_HUMAN antibody
    • PKA C beta antibody
    • PKA C-beta antibody
    • PKACB antibody
    • Prkacb antibody
    • protein kinase A catalytic subunit beta antibody
    • Protein kinase cAMP dependent catalytic beta antibody
    see all

Images

  • Anti-PKA beta (catalytic subunit) antibody (ab197047) at 1/500 dilution + SW480 cell extract

    Predicted band size: 41 kDa

  • Immunofluorescence analysis of U2OS cells, labeling PKA beta (catalytic subunit) using ab197047 at a 1/50 dilution. Blue: DAPI for nuclear staining.

References

ab197047 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab197047.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up