Overview

  • Product name
  • Description
    Rabbit polyclonal to PKA R2
  • Host species
    Rabbit
  • Specificity
    ab38949 binds to PKARIIa, but does not cross react with the other PKA regulatory subunits (PKARIa, PKARIb, PKARIIb) and can recognize native or reduced PKARIIa.
  • Tested applications
    Suitable for: ICC/IF, WB, ELISA, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
  • Immunogen

    Synthetic peptide of Rat cAMP-dependent protein kinase type II-alpha regulatory subunit. (Peptide available as ab41297.)

  • General notes
    Store diluted antibody at 4°C with 0.05% Sodium Azide as preservative.

Properties

Applications

Our Abpromise guarantee covers the use of ab38949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/1000 - 1/5000. Detects a band of approximately 55, 53 kDa (predicted molecular weight: 45 kDa).
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Function
    Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
  • Tissue specificity
    Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
  • Sequence similarities
    Belongs to the cAMP-dependent kinase regulatory chain family.
    Contains 2 cyclic nucleotide-binding domains.
  • Post-translational
    modifications
    Phosphorylated by the activated catalytic chain.
  • Cellular localization
    Cytoplasm. Cell membrane. Colocalizes with PJA2 in the cytoplasm and the cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • cAMP dependent protein kinase regulatory subunit alpha 2 antibody
    • cAMP dependent protein kinase regulatory subunit RII alpha antibody
    • cAMP dependent protein kinase type II alpha regulatory chain antibody
    • cAMP dependent protein kinase type II alpha regulatory subunit antibody
    • cAMP-dependent protein kinase type II-alpha regulatory subunit antibody
    • KAP2 antibody
    • KAP2_HUMAN antibody
    • MGC3606 antibody
    • PKR 2 antibody
    • PKR2 antibody
    • PRKA R2 antibody
    • PRKAR 2 antibody
    • PRKAR2 antibody
    • PRKAR2A antibody
    • Protein kinase A RII alpha subunit antibody
    • Protein kinase cAMP dependent regulatory type II alpha antibody
    see all

Images

  • ICC/IF image of ab38949 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38949, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ICC/IF image of ab38949 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38949, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Cederquist CT  et al. Systemic insulin sensitivity is regulated by GPS2 inhibition of AKT ubiquitination and activation in adipose tissue. Mol Metab 6:125-137 (2017). Read more (PubMed: 28123943) »
  • Ilouz R  et al. Isoform-specific subcellular localization and function of protein kinase A identified by mosaic imaging of mouse brain. Elife 6:N/A (2017). Read more (PubMed: 28079521) »
See all 8 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (hepatocytes)
Specification
hepatocytes
Treatment
20 ug/ml poly(I:C)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jul 03 2014

Answer

Thank you for your reply. The only information that we have on the specificity of the antibody is that which is listed on the datasheet, so there is no additional data that I can provide you with unfortunately. If you are going to be using the antibody in a western blot or IHC assay then as we have no images for those applications, I can offer you an Image Testing Discount. How the testing program works is, we would issue you a testing discount code for ab38949. You would then purchase the antibody at full price, test it in either western blot or IHC write an Abreview and submit an image of your results on how the antibody functioned (positive or negative). Once the Abreview has been received, the testing discount code that was originally sent to you would become active and this would allow you to receive one antibody free of charge from out catalogue. If you would be interested in this program or if there is anything else I can help you with, please let me know.

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Answer

Thank you for contacting Abcam. The immunogen sequence for ab38949 is proprietary and I don't have access to that information. It does say on the datasheet that the antibody will not cross react with the other PKA regulatory subunits (PKARIa, PKARIb, PKARIIb) and can recognize native or reduced PKARIIa. If there is anything else I can help you with, please let me know.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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