Anti-PKA R2/PKR2 antibody (ab236855)
Key features and details
- Rabbit polyclonal to PKA R2/PKR2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PKA R2/PKR2 antibody
See all PKA R2/PKR2 primary antibodies -
Description
Rabbit polyclonal to PKA R2/PKR2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human PKA R2/PKR2 aa 1-250.
Database link: P13861 -
Positive control
- WB: HeLa, MCF7 and PC-3 whole cell lysates. IHC-P: Human lymph node and glioma tissue. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236855 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/5000. Predicted molecular weight: 46 kDa.
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IHC-P |
1/20 - 1/200.
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ICC/IF |
1/50 - 1/200.
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Notes |
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WB
1/500 - 1/5000. Predicted molecular weight: 46 kDa. |
IHC-P
1/20 - 1/200. |
ICC/IF
1/50 - 1/200. |
Target
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Function
Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase. -
Tissue specificity
Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible. -
Sequence similarities
Belongs to the cAMP-dependent kinase regulatory chain family.
Contains 2 cyclic nucleotide-binding domains. -
Post-translational
modificationsPhosphorylated by the activated catalytic chain. -
Cellular localization
Cytoplasm. Cell membrane. Colocalizes with PJA2 in the cytoplasm and the cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 5576 Human
- Omim: 176910 Human
- SwissProt: P13861 Human
- Unigene: 631923 Human
- Unigene: 731573 Human
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Alternative names
- cAMP dependent protein kinase regulatory subunit alpha 2 antibody
- cAMP dependent protein kinase regulatory subunit RII alpha antibody
- cAMP dependent protein kinase type II alpha regulatory chain antibody
see all
Images
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All lanes : Anti-PKA R2/PKR2 antibody (ab236855) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 46 kDa -
Paraffin-embedded human lymph node tissue stained for PKA R2/PKR2 using ab236855 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human glioma tissue stained for PKA R2/PKR2 using ab236855 at 1/100 dilution in immunohistochemical analysis.
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HepG2 (human liver hepatocellular carcinoma cell line) cells stained for PKA R2/PKR2 (green) using ab236855 at 1/100 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab236855 has been referenced in 1 publication.
- Wei X et al. Silencing of Rab23 by siRNA inhibits ultraviolet B-induced melanogenesis via downregulation of PKA/CREB/MITF. Exp Dermatol 31:1253-1263 (2022). PubMed: 35514241