Key features and details
- Rabbit polyclonal to PKA R2/PKR2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PKA R2/PKR2 antibody
See all PKA R2/PKR2 primary antibodies
DescriptionRabbit polyclonal to PKA R2/PKR2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- WB: HeLa, MCF7 and PC-3 whole cell lysates. IHC-P: Human lymph node and glioma tissue. ICC/IF: HepG2 cells.
This product was previously labelled as PKA R2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab236855 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 46 kDa.|
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
FunctionRegulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
Tissue specificityFour types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
Sequence similaritiesBelongs to the cAMP-dependent kinase regulatory chain family.
Contains 2 cyclic nucleotide-binding domains.
modificationsPhosphorylated by the activated catalytic chain.
Cellular localizationCytoplasm. Cell membrane. Colocalizes with PJA2 in the cytoplasm and the cell membrane.
- Information by UniProt
- cAMP dependent protein kinase regulatory subunit alpha 2 antibody
- cAMP dependent protein kinase regulatory subunit RII alpha antibody
- cAMP dependent protein kinase type II alpha regulatory chain antibody
All lanes : Anti-PKA R2/PKR2 antibody (ab236855) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 46 kDa
Paraffin-embedded human lymph node tissue stained for PKA R2/PKR2 using ab236855 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human glioma tissue stained for PKA R2/PKR2 using ab236855 at 1/100 dilution in immunohistochemical analysis.
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for PKA R2/PKR2 (green) using ab236855 at 1/100 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236855 has not yet been referenced specifically in any publications.