Product nameAnti-PKA R2/PKR2 antibody [Y116]
See all PKA R2/PKR2 primary antibodies
DescriptionRabbit monoclonal [Y116] to PKA R2/PKR2
SpecificityThe antibody recognises PKA RII. It does not cross-react with other cAMP-dependent kinase.
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IPmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Cow, Human
Synthetic peptide within Human PKA R2/PKR2 aa 350-450 (C terminal). The exact sequence is proprietary.
- K562 cell lysate, HeLa cells and normal breast tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as PKA R2
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab32514 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 45 kDa).|
|IHC-P||1/100 - 1/250.|
|ICC/IF||1/100 - 1/250.|
FunctionRegulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
Tissue specificityFour types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
Sequence similaritiesBelongs to the cAMP-dependent kinase regulatory chain family.
Contains 2 cyclic nucleotide-binding domains.
modificationsPhosphorylated by the activated catalytic chain.
Cellular localizationCytoplasm. Cell membrane. Colocalizes with PJA2 in the cytoplasm and the cell membrane.
- Information by UniProt
- cAMP dependent protein kinase regulatory subunit alpha 2 antibody
- cAMP dependent protein kinase regulatory subunit RII alpha antibody
- cAMP dependent protein kinase type II alpha regulatory chain antibody
Anti-PKA R2/PKR2 antibody [Y116] (ab32514) at 1/50000 dilution + K562 cell lysate
Predicted band size: 45 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin-embedded human normal breast tissue using ab32514 at 1/100 dilution.
Immunofluorescent staining of HeLa cells using ab32514 at 1/100 dilution.
This product has been referenced in:
- Hillman P et al. Dissociation between AKAP3 and PKARII promotes AKAP3 degradation in sperm capacitation. PLoS One 8:e68873 (2013). WB ; Cow . Read more (PubMed: 23894359) »
- Gu L et al. Protein kinase A activation confers resistance to trastuzumab in human breast cancer cell lines. Clin Cancer Res 15:7196-206 (2009). IHC-P ; Human . Read more (PubMed: 19920112) »